Zheyu C, Lunan Y
Liver Transplantation Division, Department of Surgery, West China Hospital, Sichun University Medical School, Chengdu 610041, China.
Transplant Proc. 2007 Jun;39(5):1642-6. doi: 10.1016/j.transproceed.2007.02.064.
The purpose of this study was to explore the early changes of colon motility in rats after liver transplantation. Thirty Wistar rats were divided into a sham operation group (n = 10) and a liver transplantation group (n = 10 pairs). The number of stools, the contractility of muscle strips, the length of smooth muscle cells, the levels of plasma endotoxin, the morphological changes, and the expression of inducible nitric oxide synthase (iNOS) in the colon of the rats were observed in the two groups. N(6)-(iminoethyl)-L-lysine (L-NIL, a selective iNOS antagonist) was used to confirm the activity of iNOS in the contractility of colonic motility. We observed the changes of nitrogen monoxide (NO) in plasma and colon mucosa of the two groups. Results showed that the liver transplantation group compared with the sham operation group showed significantly decreased contractility of the colon with significant differences in the morphological changes in rat colon. The expression of iNOS protein and iNOSmRNA was significantly increased in the liver transplantation group. The concentrations of plasma and colon mucosa NO and the levels of endotoxin were higher among the liver transplantation group than the sham operation group (P < .05). When the strips and cells of smooth muscle from the liver transplantation group were treated with L-NIL, their contractility increased. We concluded that colon motility decreased in the rats after transplantation, which could be related to the levels of plasma endotoxin and iNOS expressing in the colon.
本研究旨在探讨大鼠肝移植后结肠运动的早期变化。将30只Wistar大鼠分为假手术组（n = 10）和肝移植组（n = 10对）。观察两组大鼠的粪便数量、肌条收缩性、平滑肌细胞长度、血浆内毒素水平、形态学变化以及结肠中诱导型一氧化氮合酶（iNOS）的表达。使用N(6)-(亚氨乙基)-L-赖氨酸（L-NIL，一种选择性iNOS拮抗剂）来证实iNOS在结肠运动收缩性中的活性。我们观察了两组大鼠血浆和结肠黏膜中一氧化氮（NO）的变化。结果显示，与假手术组相比，肝移植组大鼠结肠收缩性显著降低，结肠形态学变化存在显著差异。肝移植组中iNOS蛋白和iNOSmRNA的表达显著增加。肝移植组血浆和结肠黏膜中NO浓度以及内毒素水平均高于假手术组（P < 0.05）。当用L-NIL处理肝移植组的平滑肌条和细胞时，其收缩性增强。我们得出结论，移植后大鼠结肠运动减弱，这可能与血浆内毒素水平以及结肠中iNOS的表达有关。
