Krakow Elizabeth F, Goudar Ranjit, Petzold Elizabeth, Suvarna Shayela, Last Michael, Welsby Ian J, Ortel Thomas L, Arepally Gowthami M
Department of Medicine, Duke University Medical Center, Durham, NC, USA.
Am J Clin Pathol. 2007 Jul;128(1):150-5. doi: 10.1309/RFQK57F5QMURQ1HY.
Heparin-induced thrombocytopenia is a life threatening thrombotic disorder caused by antibodies to platelet factor 4 (PF4) and heparin. Commercial immunoassays are frequently used for the detection of PF4-heparin antibodies, and several studies have reported that higher antibody titers are more frequently associated with adverse events. It is not known if conditions involving sample preparation and/or storage affect the operational characteristics of PF4-heparin immunoassays. We compared the detection of PF4-heparin antibodies from 48 patient samples collected concordantly in serum separator tubes or tubes containing EDTA or sodium citrate. We also examined the effects of extended sample storage on whole blood collected in serum separator, EDTA, or citrate tubes at 4 degrees C for up to 96 hours on antibody detection. We noted that serum or plasma anticoagulated with sodium citrate or EDTA yielded comparable results. In addition, we could not demonstrate any significant sample deterioration after storage at 4 degrees C in any medium for up to 4 days. These findings suggest that PF4-heparin antibodies are largely insensitive to the effects of sample preparation and storage.
肝素诱导的血小板减少症是一种由针对血小板因子4（PF4）和肝素的抗体引起的危及生命的血栓形成性疾病。商业免疫测定法常用于检测PF4-肝素抗体，多项研究报告称，较高的抗体滴度更常与不良事件相关。尚不清楚涉及样本制备和/或储存的条件是否会影响PF4-肝素免疫测定的操作特性。我们比较了从在血清分离管或含有乙二胺四乙酸（EDTA）或柠檬酸钠的试管中一致采集的48份患者样本中检测PF4-肝素抗体的情况。我们还研究了将血清分离管、EDTA管或柠檬酸钠管中采集的全血在4℃下延长样本储存长达96小时对抗体检测的影响。我们注意到，用柠檬酸钠或EDTA抗凝的血清或血浆产生了可比的结果。此外，我们无法证明在任何培养基中于4℃储存长达4天后样本有任何明显变质。这些发现表明，PF4-肝素抗体在很大程度上对样本制备和储存的影响不敏感。
