Pfister Karin, Radons Jürgen, Busch Raymonde, Tidball James G, Pfeifer Michael, Freitag Lutz, Feldmann Horst-Jürgen, Milani Valeria, Issels Rolf, Multhoff Gabriele
Department for Surgery, University Hospital Regensburg, Regensburg, Germany.
Cancer. 2007 Aug 15;110(4):926-35. doi: 10.1002/cncr.22864.
Heat shock proteins (HSPs) play important roles in tumor immunity. The authors prospectively investigated the correlation between the tumor-specific Hsp70 membrane expression as an independent clinicopathological marker and overall survival in tumor entities that differ in their route of metastasis.
Hsp70 membrane expression was examined by flow cytometry in 58 colon, 19 gastric, 54 lower rectal carcinoma, and 19 squamous cell carcinoma specimens and the corresponding normal tissues at time of first diagnosis. Kaplan-Meier survival curves were analyzed to determine the relation of Hsp70 expression to the patients' prognosis.
An Hsp70 membrane-positive phenotype was found in 40% (colon), 37% (gastric), 43% (lower rectal), and 42% (squamous cell) of the analyzed tumor specimens. None of the corresponding normal tissues was found to be Hsp70 membrane-positive. In patients with colon (P = .032) and gastric (P = .045) carcinomas, an Hsp70 membrane expression correlated significantly with an improved overall survival; a negative association was seen in lower rectal (P = .085) and squamous cell carcinoma (P = .048).
The authors hypothesized that differing relations between surface expression of Hsp70 on tumor cells and clinical outcomes may reflect differences in the route of metastases. Colon and gastric carcinomas metastasize into the liver where hepatic natural killer cells may have the capacity to recognize and kill Hsp70 membrane-positive tumor cells and thus account for a better overall survival.
热休克蛋白（HSPs）在肿瘤免疫中发挥重要作用。作者前瞻性地研究了肿瘤特异性Hsp70膜表达作为一种独立的临床病理标志物与转移途径不同的肿瘤实体的总生存期之间的相关性。
在首次诊断时，通过流式细胞术检测58例结肠癌、19例胃癌、54例低位直肠癌和19例鳞状细胞癌标本及其相应正常组织中的Hsp70膜表达。分析Kaplan-Meier生存曲线以确定Hsp70表达与患者预后的关系。
在所分析的肿瘤标本中，40%（结肠癌）、37%（胃癌）、43%（低位直肠癌）和42%（鳞状细胞癌）呈现Hsp70膜阳性表型。未发现相应的正常组织呈Hsp70膜阳性。在结肠癌（P = 0.032）和胃癌（P = 0.045）患者中，Hsp70膜表达与总生存期的改善显著相关；在低位直肠癌（P = 0.085）和鳞状细胞癌（P = 0.048）中则呈负相关。
作者推测，肿瘤细胞表面Hsp70表达与临床结局之间的不同关系可能反映了转移途径的差异。结肠癌和胃癌转移至肝脏，肝脏中的自然杀伤细胞可能有能力识别并杀死Hsp70膜阳性肿瘤细胞，从而导致更好的总生存期。
