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寄生线虫分类学与生物学中电子显微镜技术综述

Review on electron microscopy in taxonomy and biology of parasitic Nemathelminthes.

作者信息

Jamjoom Manal B

机构信息

Department of Medical Parasitology, Faculty of Medicine, King AbdulAziz University, Jeddah, Saudi Arabia.

出版信息

J Egypt Soc Parasitol. 2007 Apr;37(1):87-105.

PMID:17580570
Abstract

Electron microscopy (EM) proved a very helpful means that solved a lot of information in different scientific aspects. EM is a very good tool in the hospitals and research centers. It was aimed to pile up available information on the biology in the descriptive morphology of nematodes and their immature stages by scanning (SEM) and transmission (TEM) electron microscopy. Watson (1965a, b) studied Euchromadora vulgaris and Ascaris sp. by using TEM respectively. Lee (1969) investigated the ultra-structure of Nippostrongylus brasiliensis by SEM & TEM, as well as some nematodes by TEM (Lee, 1972). The topography of the adult Baylisascaris procyonis caudal end was illustrated by Snyder (1989). Male tail relatively long, smoothly attenuated, with a small button-like or mucronate termination. Pre-anal papillae situated ventrally in 2 slightly divergent and somewhat irregularly spaced rows. Anterior and posterior to anus 2 slightly raised roughened patches consisting of several rows of small spines. Just anterior to anus along outer margin of pre-anal roughened patch, a large double medio-ventral papilla. Five pairs of post-anal papillae with first pair just posterior to anus doubled and 4 pairs more closely associated in a group near tail end. Second pair with doubled papillae; but, in a few specimens fused as if 2 single closely associated papillae. Three pair single. Fourth pair of caudal papillae phasmids and in centers of each a ringed pore-like opening. Male spicules with a highly sculptured surface with a pincher-like terminal end.

摘要

电子显微镜(EM)被证明是一种非常有用的手段,它在不同科学领域解决了大量信息。电子显微镜在医院和研究中心是一种非常好的工具。其目的是通过扫描电子显微镜(SEM)和透射电子显微镜(TEM)来积累有关线虫及其未成熟阶段描述形态学中的生物学可用信息。沃森(1965a,b)分别使用透射电子显微镜研究了普通真色线虫和蛔虫属。李(1969)通过扫描电子显微镜和透射电子显微镜研究了巴西日圆线虫的超微结构,以及通过透射电子显微镜研究了一些线虫(李,1972)。斯奈德(1989)描绘了成年浣熊贝蛔虫尾端的形貌。雄性尾部相对较长,平滑变细,末端有一个小纽扣状或短尖状的突起。肛前乳突位于腹侧,排成2排,略有分歧且间距有些不规则。肛门前后有2个略微隆起的粗糙斑块,由几排小刺组成。就在肛门前方,沿着肛前粗糙斑块的外缘,有一个大的双中腹乳突。五对肛后乳突,第一对就在肛门后方加倍,另外4对在尾端附近更紧密地聚成一组。第二对有加倍的乳突;但是,在一些标本中融合在一起,好像是2个紧密相连的单个乳突。三对单个的。第四对尾乳突为尾感器,在每个尾感器的中心有一个环状孔状开口。雄性交合刺表面有高度精细的结构,末端呈钳状。

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