Bommareddy Ajay, Arasada Bhanu L, Mathees Duane P, Dwivedi Chandradhar
South Dakota State University, Departments of Pharmaceutical Sciences, Brookings, SD 57007, USA.
J AOAC Int. 2007 May-Jun;90(3):641-6.
Lignans in flaxseed have been part of the human diet for centuries. In 1955, the isolation and structure of the lignan derivative secoisolariciresinol diglucoside (SDG) was reported. The biological role of SDG and mammalian lignan metabolites enterodiol and enterolactone was initially reported 20 years later. Experimental evidences showed the beneficial effects of lignans on breast, colon, and thyroid cancer. A modified gas chromatography/mass spectrometry (GC/MS) assay was developed for lignans in serum and colon samples of rats fed flaxseed meal. The method developed for the analysis of metabolites involves extraction and derivatization of samples and quantitative analysis by selected ion monitoring using GC/MS. The levels of lignan metabolites enterodiol and enterolactone were determined to be 0.013 and 0.23 microM in serum samples and 0.008 and 1.63 microM in colon samples.
几个世纪以来，亚麻籽中的木脂素一直是人类饮食的一部分。1955年，木脂素衍生物开环异落叶松脂醇二葡萄糖苷（SDG）的分离和结构被报道。20年后，SDG以及哺乳动物木脂素代谢产物肠二醇和肠内酯的生物学作用首次被报道。实验证据表明木脂素对乳腺癌、结肠癌和甲状腺癌具有有益作用。针对喂食亚麻籽粉的大鼠血清和结肠样本中的木脂素，开发了一种改进的气相色谱/质谱（GC/MS）测定法。所开发的用于代谢产物分析的方法包括样本的提取和衍生化，以及使用GC/MS通过选择离子监测进行定量分析。血清样本中木脂素代谢产物肠二醇和肠内酯的水平分别测定为0.013和0.23微摩尔，结肠样本中分别为0.008和1.63微摩尔。
