El-Enany Nahed, Belal Fathalla, Rizk Mohamed
University of Mansoura, Faculty of Pharmacy, Department of Analytical Chemistry, 35516, Mansoura, Egypt.
J AOAC Int. 2007 May-Jun;90(3):679-85.
A simple and sensitive kinetic method has been developed for the determination of ethamsylate (ESL) in its pharmaceutical preparations. The method is based upon oxidation of ESL with 3-methyl-2-benzothiazolinone hydrazone hydrochloride in presence of cerium (IV) ammonium sulfate at room temperature for 20 min. The absorbance of the reaction product is measured at 514 nm. The absorbance-concentration plot was rectilinear over the range of 4-30 microg/mL (r = 0.9999). The lower detection limit was 0.267 microl/mL (9.110 x 10(-6) M) and the lower quantitation limit was 0.808 microg/mL. The different experimental parameters affecting the development and stability of the reaction product were studied and optimized. The proposed method was applied to the determination of ESL in formulations, and the results obtained were in good agreement with those obtained using a reference method. The proposed method was also used for the in vitro detection of ESL in spiked human plasma at its therapeutic concentration level.
已开发出一种简单灵敏的动力学方法用于测定其药物制剂中的酚磺乙胺（ESL）。该方法基于在室温下，在硫酸铈（IV）铵存在下，酚磺乙胺与盐酸3-甲基-2-苯并噻唑啉酮腙反应20分钟。在514nm处测量反应产物的吸光度。吸光度-浓度曲线在4-30μg/mL范围内呈线性（r = 0.9999）。最低检测限为0.267μg/mL（9.110×10⁻⁶M），最低定量限为0.808μg/mL。研究并优化了影响反应产物生成和稳定性的不同实验参数。该方法应用于制剂中酚磺乙胺的测定，所得结果与参考方法的结果高度一致。该方法还用于在治疗浓度水平下加标的人血浆中酚磺乙胺的体外检测。
