Aulakh Jatinder Singh, Fekete Agnes, Malik Ashok Kumar, Mahajan Rakesh Kumar, Schmitt-Kopplin Philippe
Punjabi University, Department of Chemistry, Patiala, Punjab, India.
J AOAC Int. 2007 May-Jun;90(3):834-7.
A simple and sensitive capillary electrophoretic method with ultraviolet detection has been developed for the separation and determination of ferbam [iron(III)-dimethyldithiocarbamate], maneb [manganese(II)-ethylenebisdithiocarbamate] and zineb [zinc(II)-ethylenebisdithiocarbamate], in borate buffer, after their acidic decomposition and complexation with CDTA (trans-1,2-diaminocyclohexane-N,N,N',N'-tetraacetic acid monohydrate), as CDTA-metal complexes of Fe+3, Mn +2, and Zn+2. The determination is dependent on the pH and the nature of the buffer solutions. In this method, the detection limit (signal-to-noise ratio = 3) is 0.0013, 0.0022, and 0.0023 mM for ferbam, maneb, and zineb, respectively. The relative standard deviation for the analysis of 1 mM of each was found to be 1.5 +/- 0.2%. The method was successfully applied for the analysis of red beans and grain samples spiked with ferbam, maneb, and zineb. The applicability of capillary electrophoresis as a useful tool for the simultaneous determination and analysis of ferbam, maneb, and zineb is demonstrated.
已开发出一种简单灵敏的毛细管电泳紫外检测方法，用于分离和测定福美铁（铁（III）-二甲基二硫代氨基甲酸盐）、代森锰锌（锰（II）-亚乙基双二硫代氨基甲酸盐）和代森锌（锌（II）-亚乙基双二硫代氨基甲酸盐）。在硼酸盐缓冲液中，它们经酸性分解并与CDTA（反式-1,2-二氨基环己烷-N,N,N',N'-四乙酸一水合物）络合后，以Fe+3、Mn +2和Zn+2的CDTA-金属络合物形式存在。测定结果取决于缓冲溶液的pH值和性质。在该方法中，福美铁、代森锰锌和代森锌的检测限（信噪比 = 3）分别为0.0013、0.0022和0.0023 mM。对每种1 mM的分析，相对标准偏差为1.5 +/- 0.2%。该方法成功应用于加标了福美铁、代森锰锌和代森锌的红豆和谷物样品的分析。证明了毛细管电泳作为同时测定和分析福美铁、代森锰锌和代森锌的有用工具的适用性。
