An Ying Hui, Li Xiao Fang, Zhu Jin Xin, Shao Xing Hua, Sun Yue, Xiong Li Jun
School of Life Sciences, East China Normal University, Shanghai 200062.
Fen Zi Xi Bao Sheng Wu Xue Bao. 2007 Apr;40(2):130-6.
CAL (CAULIFLOWER) gene and AP1 (APETALA1) gene, which both belong to MADS-box transcription factors, are involved in the development of flower apical meristem. In A rabidopsis thaliana, loss of CAL and AP1 function at the same time leads to floweral meristems proliferation indeterminately, and the plants display the cauliflower phenotype. But in cauliflower (Brasscia oleracea L. var. botrytis), the BobCAL single mutant can display the cauliflower phenotype, it is appear that CAL and AP1 homolog have different function in both plants. To study the function of homozygous CAL , AP1 protein in Brassica Species, especially their regulation function in the formation of cauliflower, we took advantage of yeast-two-hybrid method to screen interaction factors of BoCAL. We got four kinds of proteins which are separately involve in phosphorylation and dephosphorylation of proteins, proteins modifying, proteins binding-site and so on. They separately have close relationship with translation, regulation pathways and signal transferring pathways and they provide clues for the study of function of BoCAL. Additionally, we checked up the relationship between some interacting factors of BoCAL and BoAP1, some known MADS-box translation factors and BoCAL and BoAP1. The results showed that BoCAL specifically interact with SnRKbeta2. BoCAL, BoAP1 both interact with SVP, similar as the homolog in Arabidopsis. While the interaction between BoCAL, BoAP1 with FLM, SOC1 and AGL24. The data here indicate the function of homozygous BoCAL and BoAP1 differ from that in Arabidopsis thaliana.
CAL（花椰菜）基因和AP1（花被1）基因均属于MADS盒转录因子，参与花顶端分生组织的发育。在拟南芥中，CAL和AP1功能同时丧失会导致花分生组织无限增殖，植株表现出花椰菜表型。但在花椰菜（Brasscia oleracea L. var. botrytis）中，BobCAL单突变体就能表现出花椰菜表型，似乎CAL和AP1同源物在这两种植物中的功能不同。为了研究芸苔属植物中纯合CAL、AP1蛋白的功能，尤其是它们在花椰菜形成中的调控功能，我们利用酵母双杂交方法筛选BoCAL的相互作用因子。我们得到了四种分别参与蛋白质磷酸化和去磷酸化、蛋白质修饰、蛋白质结合位点等的蛋白质。它们分别与翻译、调控途径和信号转导途径密切相关，为研究BoCAL的功能提供了线索。此外，我们检测了BoCAL的一些相互作用因子与BoAP1、一些已知的MADS盒翻译因子与BoCAL和BoAP1之间的关系。结果表明，BoCAL与SnRKbeta2特异性相互作用。BoCAL、BoAP1均与SVP相互作用，与拟南芥中的同源物类似。而BoCAL、BoAP1与FLM、SOC1和AGL24之间存在相互作用。此处的数据表明纯合BoCAL和BoAP1的功能与拟南芥中的不同。
