Gu Xue, Qu Qishu, Yan Chao
School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Se Pu. 2007 Mar;25(2):157-62.
Capillary electrochromatography (CEC) has attracted increasing interests in recent years. It combines the advantages of high efficiency of capillary electrophoresis (CE) with high selectivity of high performance liquid chromatography (HPLC). CEC also has a double retention mechanism of both CE and HPLC and, therefore, is suitable for simultaneous separation and analysis of neutral and charged compounds. In this paper, the preparation methods for various types of capillary columns for electrochromatography are reviewed in detail and their advantages and disadvantages are discussed.
近年来,毛细管电色谱(CEC)已引起越来越多的关注。它结合了毛细管电泳(CE)的高效性和高效液相色谱(HPLC)的高选择性的优点。CEC还具有CE和HPLC的双重保留机制,因此适用于中性和带电化合物的同时分离与分析。本文详细综述了各种类型的电色谱毛细管柱的制备方法,并讨论了它们的优缺点。