Han Ying, Xiong Zhili, Yang Chunjuan, Liu Man, Li Famei
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Se Pu. 2007 Mar;25(2):207-10.
A method was developed for the quantitative determination of protocatechuic acid in rat plasma by high performance liquid chromatography (HPLC). With added p-hydroxybenzoic acid as internal standard, the plasma samples were extracted with a solvent mixture of methanol and acetonitrile ( 1: 5, v/v). The analyte was determined by HPLC with a Diamondsil C18 column and a mobile phase of acetonitrile-water (adjusted to pH 2.5 with H3PO4, 9: 91, v/v) at a flow rate of 1. 2 mL/min. The UV detection wavelength was 260 nm. The assay exhibited a good linearity in the concentration range from 0. 050 to 3. 20 mg/L with a correlation coefficient of 0. 997 8. The limit of quantification was 0. 050 mg/L. The relative standard deviations of intra-day and inter-day determination were both less than 7. 0% and the accuracy ranged from - 1. 4% to 2. 6%. The extraction recoveries of protocatechuic acid from rat plasma samples at three concentration levels were 83. 4%, 87. 3%, and 91. 1%, respectively. The developed method was applied to a pharmacokinetic study. The main pharmacokinetic parameters in rats after a single oral dose of 10 mL/kg body weight were as follows: Cmax of (3. 16 +0. 03) microg/mL, tmax of (0. 50 +/- 0. 00) h, AU0-->t of (39. 9 +/- 5. 9) microg x mL(-1) x h, AUC-->infinity of (54. 8 +/- 8. 1) microg x mL(-1) x h and t1/2 of (3. 87 +/- 0. 25) h. The method was proved to be simple, sensitive and accurate, thus suitable for the pharmacokinetic study of protocatechuic acid in rat plasma.
建立了一种用高效液相色谱法（HPLC）定量测定大鼠血浆中原儿茶酸的方法。以对羟基苯甲酸为内标，血浆样品用甲醇和乙腈的混合溶剂（1:5，v/v）萃取。采用Diamondsil C18柱，以乙腈-水（用H3PO4调至pH 2.5，9:91，v/v）为流动相，流速为1.2 mL/min，通过HPLC测定分析物。紫外检测波长为260 nm。该测定法在0.050至3.20 mg/L的浓度范围内呈现良好的线性，相关系数为0.997 8。定量限为0.050 mg/L。日内和日间测定的相对标准偏差均小于7.0%，准确度范围为-1.4%至2.6%。原儿茶酸在大鼠血浆样品中三个浓度水平的萃取回收率分别为83.4%、87.3%和91.1%。所建立的方法应用于药代动力学研究。大鼠单次口服剂量为10 mL/kg体重后的主要药代动力学参数如下：Cmax为(3.16±0.03)μg/mL，tmax为(
