[快速气相色谱法测定食用油中未衍生化的长链脂肪酸]

[Determination of underivatized long-chain fatty acids in edible oils by fast gas chromatography].

作者信息

Meng Zhe, Wen Dawei, Liao Yiping, Liu Huwei

机构信息

College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

出版信息

Se Pu. 2007 Mar;25(2):254-7.

PMID:17580699

Abstract

A fast gas chromatography (GC) method with short micro-bore capillary column was developed for the determination of underivatized long-chain fatty acids (LCFAs) in edible oils. Baseline separation of eight kinds of LCFAs (dodecanoic acid, tetradecanoic acid, palmitic acid, oleic acid, stearic acid, eicosanic acid, erudcic acid, behenic acid) was achieved within 2 min with high resolution performance. The linear correlation coefficients for underivatized LCFAs were higher than 0. 988 and detection limits (at signal-to-noise of 3:1 ) were 2. 80 -9. 60 mg/L. The method is easy and low-cost and has the ability to achieve an accurate determination of the LCFAs in three edible oils.

摘要

建立了一种采用短微径毛细管柱的快速气相色谱（GC）方法，用于测定食用油中未衍生化的长链脂肪酸（LCFA）。在2分钟内实现了8种LCFA（十二烷酸、十四烷酸、棕榈酸、油酸、硬脂酸、二十烷酸、芥酸、山嵛酸）的基线分离，具有高分辨率性能。未衍生化LCFA的线性相关系数高于0.988，检测限（信噪比为3:1时）为2.80 - 9.60 mg/L。该方法简便、成本低，能够准确测定三种食用油中的LCFA。