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Purification of dibenzothiophene monooxygenase from a recombinant Escherichia coli.

作者信息

Gupta Nidhi, Adhikari D K, Stobdan T, Roychoudhury P K, Deb J K

机构信息

Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India.

出版信息

Biotechnol Lett. 2007 Oct;29(10):1465-8. doi: 10.1007/s10529-007-9431-5. Epub 2007 Jun 21.

DOI:10.1007/s10529-007-9431-5
PMID:17581702
Abstract

Dibenzothiophene monooxygenase is the first enzyme involved in the degradation of dibenzothiophene. This gene was expressed via the pET28a vector in E. coli and was purified in a single step using affinity chromatography. The protein was purified 39-fold with a specific activity of 38 U/mg.

摘要

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