Gupta Nidhi, Adhikari D K, Stobdan T, Roychoudhury P K, Deb J K
Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110016, India.
Biotechnol Lett. 2007 Oct;29(10):1465-8. doi: 10.1007/s10529-007-9431-5. Epub 2007 Jun 21.
Dibenzothiophene monooxygenase is the first enzyme involved in the degradation of dibenzothiophene. This gene was expressed via the pET28a vector in E. coli and was purified in a single step using affinity chromatography. The protein was purified 39-fold with a specific activity of 38 U/mg.