Chung Kyung Min, Thompson Bruce S, Fremont Daved H, Diamond Michael S
Department of Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA.
J Virol. 2007 Sep;81(17):9551-5. doi: 10.1128/JVI.00879-07. Epub 2007 Jun 20.
Previous studies have suggested that monoclonal antibodies (MAbs) to flavivirus nonstructural protein-1 (NS-1) protect against infection in mice through an Fc-gamma receptor-dependent pathway. To identify a specific mechanism, we evaluated the protective activity of anti-NS1 MAbs to WNV using mice and cells with deficiencies of specific Fc-gamma receptors. Our results suggest that only MAbs that recognize cell surface-associated NS1 trigger Fc-gamma receptor I- and/or IV-mediated phagocytosis and clearance of WNV-infected cells. These findings may be relevant for generating novel therapeutic MAbs or vaccines against flaviviruses that target the NS1 protein.
先前的研究表明，针对黄病毒非结构蛋白1（NS-1）的单克隆抗体（MAbs）通过Fc-γ受体依赖性途径保护小鼠免受感染。为了确定具体机制，我们使用缺乏特定Fc-γ受体的小鼠和细胞评估了抗NS1单克隆抗体对西尼罗河病毒（WNV）的保护活性。我们的结果表明，只有识别细胞表面相关NS1的单克隆抗体才能触发Fc-γ受体I和/或IV介导的吞噬作用以及清除WNV感染的细胞。这些发现可能与开发针对靶向NS1蛋白的黄病毒的新型治疗性单克隆抗体或疫苗相关。
