Newman Janet, Xu Jian, Willis Michael C
Molecular and Health Technologies, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.
Acta Crystallogr D Biol Crystallogr. 2007 Jul;63(Pt 7):826-32. doi: 10.1107/S0907444907025784. Epub 2007 Jun 15.
Experiments were set up to test how the crystallization drop size affects the crystallization process; in the test cases studied, increasing the drop size led to increasing numbers of crystals. Other data produced from a high-throughput automation-system run were analyzed in order to gauge the effect of replication on the success of crystallization screening. With over 40-fold multiplicity, lysozyme was found to crystallize in over half of the conditions in a standard 96-condition screen. However, despite the fact that industry-standard lysozyme was used in our tests, it was rare that we obtained crystals reproducibly; this suggests that replication whilst screening might improve the success rate of macromolecular crystallization.
开展了实验以测试结晶液滴大小如何影响结晶过程；在所研究的测试案例中，增大液滴大小会导致晶体数量增加。分析了高通量自动化系统运行产生的其他数据，以评估重复操作对结晶筛选成功率的影响。在超过40倍的重复次数下，发现溶菌酶在标准的96种条件筛选中有超过一半的条件下结晶。然而，尽管我们的测试中使用了行业标准的溶菌酶，但我们很少能可重复地获得晶体；这表明在筛选过程中进行重复操作可能会提高大分子结晶的成功率。
