Jia Yanwei, Liu Xiang-Yang
Department of Physics, National University of Singapore, 2 Science Drive 3, Singapore 117542.
J Phys Chem B. 2006 Apr 6;110(13):6949-55. doi: 10.1021/jp0536089.
A new criterion based on surface and volume diffusion kinetics was established to predict protein crystallization. Similar to the layer-by-layer crystal growth process of protein, the kinetics of the two-dimensional self-assembly of protein at the aqueous solution surface provides a convenient and reliable way to estimate the surface integration and the volume transport during protein crystallization. Both the surface and diffusion kinetics were estimated based on the protein self-assembly at the air/solution interface, which can be obtained by measuring the surface tension. A crystallization coefficient is found to provide an effective and reliable criterion to predict protein crystallization conditions. This criterion has been applied to lysozyme, concanavalin A and BSA crystallization, and it turns out to be very successful and more reliable than the second virial coefficient criterion.
建立了一种基于表面和体积扩散动力学的新准则来预测蛋白质结晶。类似于蛋白质的逐层晶体生长过程，蛋白质在水溶液表面的二维自组装动力学为估算蛋白质结晶过程中的表面整合和体积传输提供了一种便捷可靠的方法。表面和扩散动力学均基于蛋白质在空气/溶液界面的自组装来估算，这可通过测量表面张力获得。发现结晶系数为预测蛋白质结晶条件提供了一种有效且可靠的准则。该准则已应用于溶菌酶、伴刀豆球蛋白A和牛血清白蛋白的结晶，结果证明非常成功，且比第二维里系数准则更可靠。
