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通过基质辅助激光解吸/电离飞行时间质谱法分析聚甲基丙烯酸的策略

Strategies for the analysis of poly(methacrylic acid) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

作者信息

Wyatt Mark F, Schaeffer Nicolas, Tan Bien, Cooper Andrew I

机构信息

EPSRC National Mass Spectrometry Service Centre (NMSSC), School of Medicine, Swansea University, Swansea, United Kingdom.

出版信息

J Am Soc Mass Spectrom. 2007 Aug;18(8):1507-10. doi: 10.1016/j.jasms.2007.05.015. Epub 2007 May 24.

DOI:10.1016/j.jasms.2007.05.015
PMID:17583530
Abstract

We evaluated several aqueous-based sample preparation protocols for the analysis of poly(methacrylic acid) (PMAA) by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS). The sample contained a pentaerythritol tetra(3-mercaptopropionate) end-group, and was characterized in positive and negative ion modes using 2,5-dihydroxybenzoic acid (DHB) and 2,4,6-trihydroxyacetophenone (THAP) matrices. The major series observed were the M + Na species, in positive ion mode, and the M - H species, in negative ion mode. The performance of DHB and THAP matrices was comparable in positive ion mode, but THAP outperformed DHB in negative ion mode. The use of ion-exchange beads (IXB) benefited the analysis, while the addition of sodium acetate (NaOAc) or trifluoroacetic acid (TFA) proved disadvantageous in positive ion mode.

摘要

我们评估了几种基于水的样品制备方案，用于通过基质辅助激光解吸/电离飞行时间质谱（MALDI-TOFMS）分析聚甲基丙烯酸（PMAA）。样品含有季戊四醇四（3-巯基丙酸酯）端基，并使用2,5-二羟基苯甲酸（DHB）和2,4,6-三羟基苯乙酮（THAP）基质在正离子和负离子模式下进行表征。在正离子模式下观察到的主要系列是M + Na物种，在负离子模式下是M - H物种。DHB和THAP基质在正离子模式下的性能相当，但THAP在负离子模式下优于DHB。使用离子交换珠（IXB）有利于分析，而在正离子模式下添加醋酸钠（NaOAc）或三氟乙酸（TFA）被证明是不利的。

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本文引用的文献

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