Zou Tong, Liu Nan, Li Shu-de, Su Yong-chun, Man Yong, Lu Di
Department of Cardiology, Beijing Hospital, Beijing, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2007 Jun;27(6):783-6.
To investigate the role of reactive oxygen species (ROS) and the effect of vitamin E on proliferation of vascular smooth muscle cells (VSMCs) induced by homocysteine.
DNA synthesis in the VSMCs cells was measured using [3H]-thymidine incorporation assay, and the cell number determined by trypan blue method. The level of ROS in the cells was determined using DCF-DA as the fluorescence probe.
Homocysteine promoted VSMC DNA synthesis, proliferation, and ROS production. Cysteine resulted in increased ROS production in VSMCs, but had no significant effect on DNA synthesis and cell proliferation. Catalase significantly inhibited ROS production induced by homocysteine, but did not significantly inhibited homocysteine-mediated proliferation of VSMCs. While alpha-tocopherol and beta-tocopherol both suppressed increased ROS production induced by homocysteine in VSMCs, only alpha-tocopherol significantly inhibited homocysteine-mediated VSMC proliferation.
ROS is not associated with VSMC proliferation, and vitamin E-induced suppression of VSMC proliferation is probably related to protein kinase C inhibition.
探讨活性氧（ROS）的作用以及维生素E对同型半胱氨酸诱导的血管平滑肌细胞（VSMC）增殖的影响。
采用[3H] - 胸腺嘧啶核苷掺入法检测VSMC细胞中的DNA合成，并通过台盼蓝法测定细胞数量。使用DCF - DA作为荧光探针测定细胞内ROS水平。
同型半胱氨酸促进VSMC的DNA合成、增殖及ROS生成。半胱氨酸导致VSMC中ROS生成增加，但对DNA合成和细胞增殖无显著影响。过氧化氢酶显著抑制同型半胱氨酸诱导的ROS生成，但未显著抑制同型半胱氨酸介导的VSMC增殖。虽然α - 生育酚和β - 生育酚均抑制同型半胱氨酸诱导的VSMC中ROS生成增加，但只有α - 生育酚显著抑制同型半胱氨酸介导的VSMC增殖。
ROS与VSMC增殖无关，维生素E诱导的VSMC增殖抑制可能与蛋白激酶C抑制有关。
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