Mohanty S K, Gupta P K
Laser Biomedical Applications and Instrumentation Division, Raja Ramanna Centre for Advanced Technology, Indore 452013, India.
Methods Cell Biol. 2007;82:563-99. doi: 10.1016/S0091-679X(06)82020-0.
The use of laser microtools for rotation and controlled transport of microscopic biological objects and for microinjection of exogenous material in cells is discussed. We first provide a brief overview of the laser tweezers-based methods for rotation or orientation of microscopic objects. Particular emphasis is placed on the methods that are more suitable for the manipulation of biological objects, and the use of these for two-dimensional (2D) and 3D rotations/orientations of intracellular objects is discussed. We also discuss how a change in the shape of a red blood cell (RBC) suspended in hypertonic buffer leads to its rotation when it is optically tweezed. The potential use of this approach for the diagnosis of malaria is also illustrated. The use of a line tweezers having an asymmetric intensity distribution about the center of its major axis for simultaneous transport of microscopic objects, and the successful use of this approach for induction, enhancement, and guidance of neuronal growth cones is presented next. Finally, we describe laser microbeam-assisted microinjection of impermeable drugs into cells and also briefly discuss possible adverse effects of the laser trap or microbeams on cells.
本文讨论了使用激光微工具对微观生物物体进行旋转和可控运输，以及将外源物质显微注射到细胞中的应用。我们首先简要概述基于激光镊子的微观物体旋转或定向方法。特别强调更适合操纵生物物体的方法，并讨论如何将这些方法用于细胞内物体的二维（2D）和三维（3D）旋转/定向。我们还讨论了悬浮在高渗缓冲液中的红细胞（RBC）在受到光镊作用时，其形状变化如何导致其旋转。此外，还说明了这种方法在疟疾诊断中的潜在应用。接下来介绍了使用在其主轴中心具有不对称强度分布的线镊子同时运输微观物体，以及该方法在诱导、增强和引导神经元生长锥方面的成功应用。最后，我们描述了激光微束辅助将不可渗透药物显微注射到细胞中，并简要讨论了激光陷阱或微束对细胞可能产生的不利影响。
