Moreau P, Dornand J, Kaplan J G
Can J Biochem. 1975 Dec;53(12):1337-41. doi: 10.1139/o75-182.
Soybean trypsin inhibitor (SBI) was found to inhibit transformation of human lymphocytes induced by mitogens (leucoagglutinin, concanavalin A, NaIO4) or in mixed lymphocyte reaction (MLR). SBI covalently cross-linked to Sepharose beads inhibited the MLR and mitogen stimulation virtually completely. We have confirmed the work of others which showed that the synthetic anti-proteases epilson-aminocaproic acid and tosyl-L-lysyl-chloromethane (TLCK) also inhibited mitogen-induced blastogenesis and we have shown that phenylmethylsulfonylfluride was effective also; all of these agents were found to inhibit the MLR as well. SBI and TLCK were most inhibitory when added along with mitogen or when mixing allogeneic cells in a MLR; significant decrease in inhibition was noted when TLCK was added 1 h after mitogen. These data support the hypothesis that protease action at a cell surface is an essential early event common to all types of lymphocyte transformation.
大豆胰蛋白酶抑制剂（SBI）被发现可抑制由有丝分裂原（白细胞凝集素、刀豆球蛋白A、高碘酸钠）诱导的人淋巴细胞转化，或混合淋巴细胞反应（MLR）中的淋巴细胞转化。与琼脂糖珠共价交联的SBI几乎完全抑制了MLR和有丝分裂原刺激。我们证实了其他人的研究结果，即合成抗蛋白酶ε-氨基己酸和甲苯磺酰-L-赖氨酰氯甲烷（TLCK）也抑制有丝分裂原诱导的细胞分裂，并且我们还表明苯甲基磺酰氟也有效；所有这些试剂也都被发现可抑制MLR。当与有丝分裂原一起添加或在MLR中混合同种异体细胞时，SBI和TLCK的抑制作用最强；当在有丝分裂原添加1小时后添加TLCK时，抑制作用显著降低。这些数据支持了这样一种假设，即细胞表面的蛋白酶作用是所有类型淋巴细胞转化共有的一个必不可少的早期事件。
