Heal Jonathan R, Roberts Gareth W, Christie Gary, Miller Andrew D
Imperial College Genetic Therapies Centre Department of Chemistry, Flowers Building, Armstrong Road Imperial College of Science, Technology and Medicine South Kensington, London, SW72AZ, UK.
Chembiochem. 2002 Jan 4;3(1):86-92. doi: 10.1002/1439-7633(20020104)3:1<86::AID-CBIC86>3.0.CO;2-L.
Complementary peptides are coded for by the nucleotide sequence (read 5'-->3') of the complementary strand of DNA. By reading the sequence of complementary DNA in the 3'-->5' direction, alternative complementary peptides may be derived. We describe the derivation, testing and analysis of six complementary peptides designed against beta-amyloid peptide 1-40 (Abeta, 40). Data is presented to show that one peptide, designated 3' -->5' betaCP1-15, binds specifically to Abeta 1-40, and inhibits both fibrilisation and neurotoxicity in vitro. This suggests that complementary peptides could be useful leads for drug discovery, especially where diseases of protein misfolding are concerned.
互补肽由DNA互补链的核苷酸序列（从5'→3'读取）编码。通过从3'→5'方向读取互补DNA序列，可以得到不同的互补肽。我们描述了针对β-淀粉样肽1-40（Aβ40）设计的六种互补肽的推导、测试和分析。数据表明，一种名为3'→5'βCP1-15的肽与Aβ1-40特异性结合，并在体外抑制纤维化和神经毒性。这表明互补肽可能是药物发现的有用线索，特别是在涉及蛋白质错误折叠疾病的情况下。
