使用质谱法分析蛋白质复合物。

Analysis of protein complexes using mass spectrometry.

作者信息

Gingras Anne-Claude, Gstaiger Matthias, Raught Brian, Aebersold Ruedi

机构信息

Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Ave, Room 992A, Toronto, Ontario, M5G 1X5, Canada.

出版信息

Nat Rev Mol Cell Biol. 2007 Aug;8(8):645-54. doi: 10.1038/nrm2208.

DOI:10.1038/nrm2208

PMID:17593931

Abstract

The versatile combination of affinity purification and mass spectrometry (AP-MS) has recently been applied to the detailed characterization of many protein complexes and large protein-interaction networks. The combination of AP-MS with other techniques, such as biochemical fractionation, intact mass measurement and chemical crosslinking, can help to decipher the supramolecular organization of protein complexes. AP-MS can also be combined with quantitative proteomics approaches to better understand the dynamics of protein-complex assembly.

摘要

亲和纯化与质谱分析（AP-MS）的多功能组合最近已应用于许多蛋白质复合物和大型蛋白质相互作用网络的详细表征。AP-MS与其他技术（如生化分级分离、完整质量测量和化学交联）的结合，有助于解析蛋白质复合物的超分子组织。AP-MS还可以与定量蛋白质组学方法相结合，以更好地理解蛋白质复合物组装的动态过程。