Alebiowu G, Femi-Oyewo M N, Elujoba A A, Ojo O S
Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
J Herb Pharmacother. 2007;7(1):1-12.
Suspensions of the powdered leaf of Datura metel L. and Datura stramonium L. (Solanaceae) were administered by intubation at different doses: 0.125 mg/kg, 0.250 mg/kg, 0.500 mg/kg, 1.000 mg/kg, 1.224 mg/kg and 1.400 mg/kg to virgin female albino mice and were observed daily. After two weeks, the mice were sacrificed and the liver, kidney and intestine were removed, preserved in 10% formalin solution and embedded in paraffin wax. Tissues from these organs were stained for assessment of tissue morphology. Pathological changes observed at 1.224 mg/kg and 1.400 mg/kg (LD100) were irreversible. At all other dose levels, there were reversible changes in the liver, kidney and intestine. Generally, D. metel-treated mice showed less anatomical abnormalities than D. stramonium-treated mice. Hence, D. metel could serve as a substitute for D. stramonium in drug development.
将曼陀罗叶粉末和洋金花叶粉末（茄科）的悬浮液以不同剂量：0.125毫克/千克、0.250毫克/千克、0.500毫克/千克、1.000毫克/千克、1.224毫克/千克和1.400毫克/千克经插管给予未交配的雌性白化小鼠，并每日进行观察。两周后，处死小鼠，取出肝脏、肾脏和肠道，保存在10%福尔马林溶液中，然后包埋在石蜡中。对这些器官的组织进行染色以评估组织形态。在1.224毫克/千克和1.400毫克/千克（LD100）剂量下观察到的病理变化是不可逆的。在所有其他剂量水平下，肝脏、肾脏和肠道出现了可逆性变化。一般来说，用曼陀罗处理的小鼠比用洋金花处理的小鼠表现出的解剖学异常更少。因此，在药物开发中，曼陀罗可以作为洋金花的替代品。
