Aumann Lars, Stroehlein Guido, Morbidelli Massimo
ETH Zurich, Institute for Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, Hoenggerberg, HCI, 8093 Zurich, Switzerland.
Biotechnol Bioeng. 2007 Dec 1;98(5):1029-42. doi: 10.1002/bit.21529.
The novel "multicolumn countercurrent solvent gradient purification" (MCSGP) process has been modeled for the purification of a polypeptide mixture characterized by a strong non-linear competitive adsorption isotherm. As a model system, the purification of an industrial polypeptide mixture containing 46% of the hormone calcitonin has been selected. The many impurities contained in the mixture have been lumped into three key impurities, which are selected as the ones eluting closer to the main component. The simulation model allows for a better understanding of the complex operating behavior of the multicolumn system, which has been experimentally investigated in a previous work. Through a systematic parametric analyses of the model behavior, the main operating parameters controlling the process performance in terms of purity and yield are investigated. The study of internal liquid and adsorbed phase concentration profiles along the unit for the different operating conditions allow elucidating the working principle of the new separation process. It is found that the MCSGP unit achieves much higher yields for a given product purity than the corresponding single-column batch units.
新型“多柱逆流溶剂梯度纯化”（MCSGP）工艺已被用于模拟纯化具有强非线性竞争吸附等温线特征的多肽混合物。作为一个模型系统，已选择对含有46%降钙素激素的工业多肽混合物进行纯化。混合物中所含的许多杂质已被归为三种关键杂质，这些杂质被选为洗脱时更接近主要成分的杂质。该模拟模型有助于更好地理解多柱系统复杂的操作行为，此前的一项工作已对该系统进行了实验研究。通过对模型行为进行系统的参数分析，研究了在纯度和产率方面控制工艺性能的主要操作参数。对不同操作条件下沿装置的内部液相和吸附相浓度分布进行研究，有助于阐明这种新型分离工艺的工作原理。研究发现，对于给定的产品纯度，MCSGP装置比相应的单柱间歇装置具有更高的产率。
