Dejarkom Surachai, Kunathikom Somboon
Department of Obstetrics and Gynecology, Faculty of Medicine Siriraj Hospital, Bangkok 10700, Thailand.
J Med Assoc Thai. 2007 May;90(5):852-6.
The present study was designed to determine the effect of the freeze-thawing procedure, computer controlled rate freezing and duration for six months, on human sperm chromatin (assessed by acridine orange test), vitality, motility, and morphology.
Experimental study
Twenty semen samples were obtained from patients attending the infertility unit. The semen analysis was measured according to WHO criteria. Sperm morphology was evaluated by strict Kruger criteria and sperm chromatin were detected by acridine orange test. After semen analysis, each sample was mixed with cryoprotectant and divided into straw. The straw was frozen with computer controlled rate freezing method After 6 months of cryostorage, semen samples were thawed and then the semen was analyed, and sperm chromatin and morphology were determined.
After six months of cryostorage, the mean percentage of normal sperm chromatin decreased significantly (87.3 +/- 9.0 vs. 51.9 +/- 27.4, p < 0.001). Vitality, motility, and normal morphology of sperm decreased significantly (78.7 +/- 1.9 vs. 32.8 + 10.8, 52.6 + 1.9 vs. 24.1 +/- 10.9 and 21.4 +/- 4.3 vs. 18.0 +/- 4.4 respectively, p < 0.001).
The computer controlled rate freezing of sperm for six months and thawing process significantly decreased normal sperm chromatin, vitality, motility, and normal morphology.
本研究旨在确定冻融程序、计算机控制速率冷冻以及六个月的保存时长对人类精子染色质（通过吖啶橙试验评估）、活力、运动能力和形态的影响。
实验研究
从不孕症科室的患者处获取20份精液样本。根据世界卫生组织标准进行精液分析。精子形态按照严格的克鲁格标准进行评估，精子染色质通过吖啶橙试验检测。精液分析后，将每个样本与冷冻保护剂混合并分装到细管中。细管用计算机控制速率冷冻法进行冷冻。冷冻保存6个月后，将精液样本解冻，然后再次进行精液分析，并测定精子染色质和形态。
冷冻保存6个月后，正常精子染色质的平均百分比显著下降（87.3±9.0对51.9±27.4，p<0.001）。精子的活力、运动能力和正常形态显著下降（分别为78.7±1.9对32.8 + 10.8、52.6 + 1.9对24.1±10.9以及21.4±4.3对18.0±4.4，p<0.001）。
精子计算机控制速率冷冻6个月及解冻过程显著降低了正常精子染色质、活力、运动能力和正常形态。
