人皮肤良性和非典型痣、黑色素瘤及黑色素瘤转移灶中肿瘤浸润性CD4+CD25+FOXP3+调节性T细胞的评估

Evaluation of tumour-infiltrating CD4+CD25+FOXP3+ regulatory T cells in human cutaneous benign and atypical naevi, melanomas and melanoma metastases.

作者信息

Mourmouras V, Fimiani M, Rubegni P, Epistolato M C, Malagnino V, Cardone C, Cosci E, Nisi M C De, Miracco C

机构信息

Department of Human Pathology and Oncology, Section of Pathological Anatomy, University of Siena, Siena 53100, Italy.

出版信息

Br J Dermatol. 2007 Sep;157(3):531-9. doi: 10.1111/j.1365-2133.2007.08057.x. Epub 2007 Jun 26.

DOI:10.1111/j.1365-2133.2007.08057.x

PMID:17596146

Abstract

BACKGROUND

CD4+CD25+FOXP3+ regulatory T cells (Tregs) are thought to induce immunotolerance in melanoma. They have not yet been investigated in the entire spectrum of melanocytic cutaneous lesions within a tumour site.

OBJECTIVES

To evaluate CD4+CD25+FOXP3+ Tregs among tumour-infiltrating lymphocytes in cutaneous melanocytic lesions.

METHODS

We analysed 128 lesions (10 benign junctional common naevi, 10 benign compound common naevi, 10 compound Spitz naevi, 10 junctional atypical naevi, 20 compound atypical naevi, 20 radial growth phase melanomas, 30 vertical growth phase melanomas and 18 melanoma metastases). Tregs were identified by CD25-FOXP3 double immunostains.

RESULTS

This study indicates that CD4+/CD25+FOXP3+ Tregs are present in all groups of lesions. Junctional atypical naevi, compound atypical naevi and radial growth phase melanomas showed the highest percentages of CD4+CD25+FOXP3+ Tregs (junctional atypical naevi vs. junctional common naevi, compound common naevi, compound Spitz naevi, melanoma metastases: P < 0.0001; junctional atypical naevi vs. vertical growth phase melanomas: P = 0.001; compound atypical naevi vs. junctional common naevi, compound common naevi: P < 0.0001; compound atypical naevi vs. compound Spitz naevi, melanoma metastases: P = 0.002; compound atypical naevi vs. vertical growth phase melanomas: P = 0.02; radial growth phase melanomas vs. junctional common naevi, compound common naevi, compound Spitz naevi, melanoma metastases: P < 0.0001; radial growth phase melanomas vs. vertical growth phase melanomas: P = 0.008).

CONCLUSIONS

The strong prevalence of CD25+FOXP3+ Tregs both in junctional and compound atypical naevi and radial growth phase melanomas, suggests that they induce immunotolerance early during melanoma genesis, favouring melanoma growth. Their evaluation within a tumour site could be useful for prognostic and therapeutic purposes.

摘要

背景

CD4+CD25+FOXP3+调节性T细胞（Tregs）被认为可诱导黑色素瘤产生免疫耐受。然而，尚未在肿瘤部位的整个黑素细胞性皮肤病变范围内对其进行研究。

目的

评估皮肤黑素细胞性病变中肿瘤浸润淋巴细胞内的CD4+CD25+FOXP3+ Tregs。

方法

我们分析了128个病变（10个良性交界性普通痣、10个良性复合性普通痣、10个复合性Spitz痣、10个交界性非典型痣、20个复合性非典型痣、20个放射状生长期黑色素瘤、30个垂直生长期黑色素瘤和18个黑色素瘤转移灶）。通过CD25-FOXP3双重免疫染色鉴定Tregs。

结果

本研究表明，所有病变组中均存在CD4+/CD25+FOXP3+ Tregs。交界性非典型痣、复合性非典型痣和放射状生长期黑色素瘤中CD4+CD25+FOXP3+ Tregs的百分比最高（交界性非典型痣与交界性普通痣、复合性普通痣、复合性Spitz痣、黑色素瘤转移灶相比：P < 0.0001；交界性非典型痣与垂直生长期黑色素瘤相比：P = 0.001；复合性非典型痣与交界性普通痣、复合性普通痣相比：P < 0.0001；复合性非典型痣与复合性Spitz痣、黑色素瘤转移灶相比：P = 0.002；复合性非典型痣与垂直生长期黑色素瘤相比：P = 0.02；放射状生长期黑色素瘤与交界性普通痣、复合性普通痣、复合性Spitz痣、黑色素瘤转移灶相比：P < 0.0001；放射状生长期黑色素瘤与垂直生长期黑色素瘤相比：P = 0.008）。