Hirokawa K, Laing R A, Oak S S, Levy S, Majima Y
Department of Ophthalmology, Fujita Health University, School of Medicine, Toyoake, Japan.
Nippon Ganka Gakkai Zasshi. 1991 Nov;95(11):1057-64.
The metabolic and functional connection between corneal endothelial cells is thought to depend on the transfer of small molecules via gap junctions, as has been reported for other tissues. Cell-to-cell communication in the corneal endothelium was studied by monitoring the spread of fluorescence following the direct injection of Lucifer Yellow CH into single endothelial cells of the excised rabbit cornea. The image of the endothelial cells was observed during the injection and post-injection periods using specular microscopy and fluorescence microscopy. In the fresh cornea, the dye transferred readily from the injected endothelial cell to its neighbors. In damaged cells, dye transfer was slower or did not occur. After 5 hours of incubation in tissue culture medium 199, the specular microscopic image degraded, lowered cell membrane potential and decreased dye transfer rate were measured. This study showed that in normal corneal endothelial cells there were efficient cell communication channels between neighboring cells, and as the endothelium becomes less viable, cell communication is inhibited.
角膜内皮细胞之间的代谢和功能联系被认为依赖于小分子通过缝隙连接的传递，其他组织也有相关报道。通过将荧光素黄CH直接注入离体兔角膜的单个内皮细胞后监测荧光的扩散，研究了角膜内皮中的细胞间通讯。在注射期间和注射后，使用镜面显微镜和荧光显微镜观察内皮细胞的图像。在新鲜角膜中，染料很容易从注射的内皮细胞转移到其相邻细胞。在受损细胞中，染料转移较慢或不发生。在199组织培养基中孵育5小时后，镜面显微镜图像退化，测量到细胞膜电位降低和染料转移速率下降。这项研究表明，在正常角膜内皮细胞中，相邻细胞之间存在有效的细胞通讯通道，并且随着内皮细胞活力降低，细胞通讯受到抑制。
