Kondo Hidehiro, Yeu Tzeh Andrew Gung, Hirono Ikuo, Aoki Takashi
Laboratory of Genome Science, Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato, Tokyo 108-8477, Japan.
Fish Shellfish Immunol. 2007 Nov;23(5):1089-94. doi: 10.1016/j.fsi.2007.05.002. Epub 2007 May 18.
A novel C-type lectin of Japanese flounder (JFCTL) was identified among Japanese flounder liver EST. Of 2083 of Japanese flounder liver ESTs in the GenBank database, 48 were identical to JFCTL, suggesting that JFCTL is abundantly produced in liver. JFCTL cDNA contains an open reading frame of 642 bp encoding 214 amino acids. A C-type lectin domain was conserved in the deduced amino acid sequence. The protein showed homology to transmembrane-type C-type lectin receptors rather than the soluble C-type lectin so far reported, but it was predicted to be a secreted protein. Transcription of JFCTL was restricted to the liver. Although the mRNA was not induced after the treatment of fish with formalin-killed Edwardsiella tarda, expression was higher at 20 degrees C than at 10 degrees C.
在牙鲆肝脏EST中鉴定出一种新型牙鲆C型凝集素（JFCTL）。在GenBank数据库中的2083条牙鲆肝脏EST中，有48条与JFCTL相同，这表明JFCTL在肝脏中大量产生。JFCTL cDNA包含一个642 bp的开放阅读框，编码214个氨基酸。在推导的氨基酸序列中，C型凝集素结构域是保守的。该蛋白与跨膜型C型凝集素受体具有同源性，而非迄今报道的可溶性C型凝集素，但预计它是一种分泌蛋白。JFCTL的转录仅限于肝脏。虽然在用福尔马林灭活的迟缓爱德华氏菌处理鱼后，mRNA未被诱导，但在20℃时的表达高于10℃时。
