Wang S L, Jacober L, Wu-Wang C Y, Liu J, Slomiany A, Slomiany B L
Research Center, New Jersey Dental School, University of Medicine and Dentistry of New Jersey, Newark 07103-2400.
Biomed Biochim Acta. 1991;50(7):879-84.
The effect of ethanol on buccal mucosal epidermal growth factor receptors was investigated. The buccal mucosal cells from adult rats were incubated in DMEM at 4 degrees C and 37 degrees C in the presence of various concentrations (0-5%) of ethanol and then assayed for EGF receptor binding using [125I]-EGF. The results of binding analysis showed that the cells incubated in the presence of ethanol displayed diminished [125I]-EGF binding. The receptor binding capacity was dependent upon ethanol concentration and was more pronounced at 4 degrees C. A 30% decrease in receptor binding occurred with 1% ethanol, 34% with 2.5% ethanol, while 5% ethanol caused a 42% decrease in binding. Scatchard analysis suggested that the decrease of [125I]-EGF binding to the buccal mucosal cells pre-incubated with ethanol was due to a decrease of the high affinity binding site (Bmax). The association constant of the high affinity EGF receptor remained unchanged. The results suggest that ethanol due to its untoward effect on the receptor binding of salivary GF interferes with the process of epithelial renewal, thus leading to weakening of the mucosal defense perimeter of soft oral tissue and the onset of dysplasia.
研究了乙醇对颊黏膜表皮生长因子受体的影响。将成年大鼠的颊黏膜细胞在含有不同浓度（0 - 5%）乙醇的DMEM中于4℃和37℃孵育，然后使用[125I]-表皮生长因子（EGF）检测EGF受体结合情况。结合分析结果显示，在乙醇存在下孵育的细胞表现出[125I]-EGF结合减少。受体结合能力取决于乙醇浓度，在4℃时更为明显。1%乙醇导致受体结合减少30%，2.5%乙醇导致减少34%，而5%乙醇导致结合减少42%。Scatchard分析表明，与乙醇预孵育的颊黏膜细胞[125I]-EGF结合减少是由于高亲和力结合位点（Bmax）减少所致。高亲和力EGF受体的结合常数保持不变。结果表明，乙醇因其对唾液生长因子受体结合的不良影响，干扰上皮更新过程，从而导致口腔软组织黏膜防御屏障减弱和发育异常的发生。
