Shahtaheri Seyed Jamaleddin, Khadem Monireh, Golbabaei Farideh, Rahimi-Froushan Abbas, Ganjali Mohammad Reza, Norouzi Parviz
School of Public Health, Center for Environmental Research, Medical Sciences/University of Tehran, Tehran, I.R. Iran.
Int J Occup Saf Ergon. 2007;13(2):137-45. doi: 10.1080/10803548.2007.11076719.
Lead is an important constituent widely used in different industrial processes. For evaluation of workers' exposure to trace toxic metal of Pb (II), solid-phase extraction (SPE) was optimized. SPE using mini columns filled with XAD-4 resin was developed with regard to sample pH, ligand concentration, loading flow rate, elution solvent, sample volume, elution volume, the amount of resins, and sample matrix interferences. Lead ions were retained on a solid sorbent and then eluted, followed by a simple determination of analytes with flame atomic absorption spectrometery. The obtained recoveries of metal ions were greater than 92%. This method was validated with 3 different pools of spiked urine samples; it showed a good reproducibility over 6 consecutive days as well as 6 within-day experiments. This optimized method can be considered successful in simplifying sample preparation for a trace residue analysis of lead in different matrices when evaluating occupational and environmental exposures is required.
铅是一种在不同工业过程中广泛使用的重要成分。为了评估工人对痕量有毒金属铅（II）的接触情况，对固相萃取（SPE）进行了优化。针对样品pH值、配体浓度、进样流速、洗脱溶剂、样品体积、洗脱体积、树脂用量和样品基质干扰等因素，开发了使用填充XAD - 4树脂的微型柱的固相萃取方法。铅离子保留在固体吸附剂上，然后进行洗脱，随后用火焰原子吸收光谱法对分析物进行简单测定。金属离子的回收率大于92%。该方法用3种不同的加标尿液样本池进行了验证；在连续6天以及6次日内实验中均显示出良好的重现性。当需要评估职业和环境暴露时，这种优化方法可被认为成功简化了不同基质中铅痕量残留分析的样品制备过程。
