Cunin Frédérique, Milhiet Pierre-Emmanuel, Anglin Emily, Sailor Michael J, Espenel Cédric, Le Grimellec Christian, Brunel Daniel, Devoisselle Jean-Marie
Institut Charles Gerhardt, UMR 5253 CNRS/ENSCM/UM2/UM1, Ecole Nationale Supérieure de Chimie de Montpellier, France.
Ultramicroscopy. 2007 Oct;107(10-11):1048-52. doi: 10.1016/j.ultramic.2007.02.041. Epub 2007 May 5.
Reconstituting artificial membranes for in vitro studies of cell barrier mechanisms and properties is of major interest in biology. Here, artificial membranes supported on porous silicon photonic crystal reflectors are prepared and investigated. The materials are of interest for label-free probing of supported membrane events such as protein binding, molecular recognition, and transport. The porous silicon substrates are prepared as multilayered films consisting of a periodically varying porosity, with pore dimensions of a few nanometers in size. Planar phospholipid bilayers are deposited on the topmost surface of the oxidized hydrophilic mesoporous silicon films. Atomic force microscopy provides evidence of continuous bilayer deposition at the surface, and optical measurements indicate that the lipids do not significantly infiltrate the porous region. The presence of the supported bilayer does not obstruct the optical spectrum from the porous silicon layer, suggesting that the composite structures can act as effective optical biosensors.
重建人工膜用于细胞屏障机制和特性的体外研究是生物学中的一个重要研究方向。在此，制备并研究了支撑在多孔硅光子晶体反射器上的人工膜。这些材料对于无标记探测支撑膜上的事件（如蛋白质结合、分子识别和转运）具有重要意义。多孔硅基底被制备成具有周期性变化孔隙率的多层膜，孔隙尺寸为几纳米。平面磷脂双层沉积在氧化的亲水中介孔硅膜的最顶层表面。原子力显微镜提供了表面连续双层沉积的证据，光学测量表明脂质不会显著渗入多孔区域。支撑双层的存在不会阻碍来自多孔硅层的光谱，这表明复合结构可以作为有效的光学生物传感器。
