Tantawi Khalid Hasan, Cerro Ramon, Berdiev Bakhrom, Martin M Elena Diaz, Montes Francisco Javier, Patel Darayas, Williams John D
Department of Electrical and Computer Engineering, University of Alabama in Huntsville, Huntsville, AL 35899, USA.
J Med Eng Technol. 2013 Jan;37(1):28-34. doi: 10.3109/03091902.2012.733056.
This article investigates a device made from a porous silicon structure supporting a lipid bilayer membrane (LBM)fused with Epithelial Sodium Channel protein. The electrochemically-fabricated porous silicon template had pore diameters in the range 0.2~2 µm. Membranes were composed of two synthetic phospholipids: 1,2-diphytanoyl-sn-glycero-3-phosphoserine and 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine. The LBMwas formed by means of the Langmuir-Blodgett and Langmuir-Schaefer techniques, at a monolayer surface tension of 26 m Nm(-1) in room temperature and on a deionized water subphase, which resulted in an average molecular area of 0.68-0.73 nm(2). Fusion of transmembrane protein was investigated using Atomic Force Microscopy. Initial atomic force microscopy results demonstrate the ability to support lipid bilayers fused with transmembrane proteins across a porous silicon substrate. However, more control of the membrane's surface tension using traditional membrane fusion techniques is required to optimize protein incorporation.
本文研究了一种由多孔硅结构制成的装置，该结构支撑着与上皮钠通道蛋白融合的脂质双分子层膜（LBM）。通过电化学方法制备的多孔硅模板的孔径范围为0.2至2微米。膜由两种合成磷脂组成：1,2 - 二植烷酰 - sn - 甘油 - 3 - 磷酸丝氨酸和1,2 - 二植烷酰 - sn - 甘油 - 3 - 磷酸乙醇胺。LBM是通过朗缪尔 - 布洛杰特（Langmuir - Blodgett）和朗缪尔 - 谢弗（Langmuir - Schaefer）技术形成的，在室温下于去离子水亚相上，单层表面张力为26 m Nm(-1)，这导致平均分子面积为0.68 - 0.73 nm(2)。使用原子力显微镜研究跨膜蛋白的融合。最初的原子力显微镜结果表明，该装置能够在多孔硅基底上支撑与跨膜蛋白融合的脂质双分子层。然而，需要使用传统的膜融合技术对膜的表面张力进行更多控制，以优化蛋白质的掺入。
