Davis Richard C, Jin Angela, Rosales Melenie, Yu Suzanne, Xia Xiaoyu, Ranola Kathleen, Schadt Eric E, Lusis Aldons J
Department of Microbiology, Immunology and Molecular Genetics, University of California at Los Angeles, Los Angeles, CA 90095-1679, USA.
Genomics. 2007 Sep;90(3):306-13. doi: 10.1016/j.ygeno.2007.05.009. Epub 2007 Jun 27.
We previously reported the construction of two sets of heterozygous congenic strains spanning the mouse genome. For both sets, C57BL/6J was employed as the background strain while DNA from either DBA/2 or CAST/Ei was introgressed to form the congenic region. We have subsequently bred most of these strains to produce homozygous breeding stocks. Here, we report the characterization of the strain set based on CAST/Ei. CAST/Ei is the most genetically distant strain within the Mus mus species and many trait variations relevant to common diseases have been identified in CAST/Ei mice. Despite breeding difficulties for some congenic regions, presumably due to incompatible allelic variations between CAST/Ei and C57BL/6, the resulting congenic strains cover about 80% of the autosomal chromosomes and will be useful as a resource for the further analysis of quantitative trait loci between the strains.
我们之前报道了构建两套跨越小鼠基因组的杂合近交系。对于这两套近交系，均以C57BL/6J作为背景品系，同时导入来自DBA/2或CAST/Ei的DNA以形成近交区域。随后，我们繁育了这些品系中的大多数以产生纯合育种群体。在此，我们报道基于CAST/Ei的品系组的特征。CAST/Ei是小家鼠物种中遗传距离最远的品系，并且在CAST/Ei小鼠中已鉴定出许多与常见疾病相关的性状变异。尽管某些近交区域的繁育存在困难，推测是由于CAST/Ei和C57BL/6之间等位基因变异不兼容所致，但最终得到的近交系覆盖了约80%的常染色体，将作为进一步分析品系间数量性状位点的资源。
