Golubovic S, Jankovic M
Institute for the Application of Nuclear Energy-INEP, University of Belgrade, Belgrade-Zemun, Serbia.
J BUON. 2007 Apr-Jun;12(2):269-75.
Carcinoembryonic antigen (CEA) is a highly glycosylated molecule, expressed in both normal and pathological conditions. This study was aimed at isolation and glycobiochemical characterization of human salivary CEA, and comparison of its structural properties with those of cancer-derived CEA.
CEA was isolated by immunoaffinity chromatography on an anti-CEA IgG-Sepharose 4B column. The isolated antigen was characterized by SDSPAGE, gel filtration on Sepharose 4B and lectin-affinity chromatography using a panel of 6 plant lectins.
The isolated salivary CEA had a molecular mass of 180 kD and retained its structural and antigenic properties. Lectin-affinity chromatography indicated pronounced microheterogeneity of both salivary and cancer-derived CEA. Comparison of the lectin-binding patterns demonstrated lower relative amounts of distinct Phaseolus vulgaris agglutinin-reactive and Pisum sativum agglutinin-reactive glycoforms in salivary CEA than in cancer-derived CEA.
The observed microheterogeneity of salivary CEA could have both functional and clinical relevance in relation to normal as well as to pathological oral physiology.
癌胚抗原（CEA）是一种高度糖基化的分子，在正常和病理条件下均有表达。本研究旨在分离人唾液CEA并对其进行糖生物化学表征，并将其结构特性与癌源CEA的结构特性进行比较。
通过在抗CEA IgG-Sepharose 4B柱上进行免疫亲和层析分离CEA。通过SDS-PAGE、Sepharose 4B凝胶过滤以及使用6种植物凝集素的凝集素亲和层析对分离出的抗原进行表征。
分离出的唾液CEA分子量为180 kD，并保留了其结构和抗原特性。凝集素亲和层析表明唾液CEA和癌源CEA均存在明显的微观异质性。凝集素结合模式的比较表明，唾液CEA中明显的菜豆凝集素反应性和豌豆凝集素反应性糖型的相对含量低于癌源CEA。
观察到的唾液CEA微观异质性可能在正常以及病理口腔生理学方面具有功能和临床相关性。
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