Liu Guang Jie, Wang Jian Ping, Xiao Jian Chun, Zhao Zhi Wei, Zheng Yong Tang
Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
Cell Mol Immunol. 2007 Jun;4(3):203-8.
HIV-1 p24 detection provides a means to aid the early diagnosis of HIV-1 infection, track the progression of disease and assess the efficacy of antiretroviral therapy. In the present study, three monoclonal antibodies (mAbs) p3JB9, p5F1 and p6F4 against HIV-1 p24 were generated. All mAbs could detect p24 of HIV-1IIIB, HIV-1Ada-M, HIV-174V mAbs p5F1 and p6F4 could detect HIV-1KM018, while p3JB9 could not. Three mAbs did not react with HIV-2ROD, HIV-2CBL-20 and SIVagmTYO-1. The recognized epitope of p5F1 was located on the Gag amino acid region DCKTILKALGPAATLEEMMTAC. The p5F1 was used to establish a modified sandwich ELISA with rabbit anti-p24 serum and showed good specificity and high sensitivity, which has been used to measure HIV-1 p24 antigen levels in research.
HIV-1 p24检测为艾滋病病毒1型（HIV-1）感染的早期诊断、疾病进展跟踪以及抗逆转录病毒疗法疗效评估提供了一种手段。在本研究中，制备了三种针对HIV-1 p24的单克隆抗体（mAb）p3JB9、p5F1和p6F4。所有单克隆抗体均能检测出HIV-1IIIB、HIV-1Ada-M、HIV-174V的p24；单克隆抗体p5F1和p6F4能检测出HIV-1KM018，而p3JB9不能。三种单克隆抗体均不与HIV-2ROD、HIV-2CBL-20和猴免疫缺陷病毒（SIV）agmTYO-1发生反应。p5F1识别的表位位于Gag氨基酸区域DCKTILKALGPAATLEEMMTAC。利用p5F1与兔抗p24血清建立了改良夹心酶联免疫吸附测定法（ELISA），该方法具有良好的特异性和高灵敏度，已用于研究中HIV-1 p24抗原水平的检测。
