Liang Xianwei, Zhang Xiufang, Yang Bingzhuang, Cheng Mingtang, Huang Fenxiang, Pang Chunying, Qing Guangsheng, Liao Chaohui, Wei Shengju, Senatore Elena M, Bella Antonino, Presicce Giorgio A
Guangxi Buffalo Research Institute, Chinese Academy of Agriculture Sciences, Nanning 530001, P.R. China.
Reprod Fertil Dev. 2007;19(5):670-6. doi: 10.1071/rd07048.
The main objective of this study was to compare pregnancy and calving rates following transfer of in-vitro-produced fresh river and F1 (river x swamp) buffalo embryos in recipients synchronised by Ovsynch protocol or following natural oestrus. River embryos were produced from cumulus-oocyte complexes (COCs) derived by ovum pick up (OPU) on 40 Murrah and Nili-Ravi donor buffaloes over a twice-weekly collection schedule for 120 single OPUs. F1 embryos were produced by fertilisation of swamp COCs recovered from abattoir ovaries coincubated with river sperm cells. Both groups of embryos were produced following the same protocol for in vitro production. With regard to the OPU source of COCs, 923 antral follicles were punctured and 647 COCs were recovered (70%). From 462 selected COCs for IVM, 257 (55.6%) cleaved zygotes were recorded leading to 93 blastocysts (20.1%). In total, 590 swamp COCs were aspirated from abattoir ovaries and 476 were selected for IVM leading to 270 (56.7%) cleaved zygotes and resulting in 137 blastocysts (28.8%). River and F1 embryos were transferred between Day 6 to 7 of in vitro development, corresponding to blastocyst-expanding blastocyst, into F1 recipients synchronised by Ovsynch and swamp buffaloes following natural oestrus, respectively, each of them receiving two embryos. According to palpation per rectum of the ovaries at the time of embryo transfer, 26 of the 47 (55.3%) F1 recipients synchronised by Ovsynch were considered suitable for transfer, resulting in seven pregnancies (26.9%) and four calvings (15.3%) owing to three abortions occurring between 2 and 3 months of pregnancy. In total, 29 swamp recipients following natural oestrus were judged suitable as recipients, resulting in 12 pregnancies (41.4%) and 10 calvings (34.5%) owing to two abortions at 2 and 3 months of gestation respectively. Pregnancy and calving rates following transfer of river and F1 embryos were similar. Likewise, weight at birth of calves derived from transfer of river and F1 embryos was not different: 30.5 +/- 1.4 and 32.9 +/- 2.4 respectively. Pregnancy and calving rates following AI in a group of river and swamp buffaloes considered for reference in this study were similar to recipients carrying in-vitro-produced embryos. Collectively, no apparent postnatal complications were recorded in resulting live calves.
本研究的主要目的是比较经Ovsynch方案同步化处理的受体或自然发情后的受体，在移植体外生产的新鲜河流型和F1（河流型×沼泽型）水牛胚胎后的妊娠率和产犊率。河流型胚胎由40头摩拉水牛和尼里-拉菲水牛供体经每周两次的采卵（OPU），共进行120次单次OPU采集获得的卵丘-卵母细胞复合体（COC）生产而来。F1胚胎通过将从屠宰场卵巢回收的沼泽型COC与河流型精子细胞共同孵育受精产生。两组胚胎均按照相同的体外生产方案生产。关于COC的OPU来源，穿刺了923个窦状卵泡，回收了647个COC（70%）。从462个选择用于体外成熟（IVM）的COC中，记录到257个（55.6%）受精卵分裂，产生93个囊胚（20.1%）。总共从屠宰场卵巢抽吸了590个沼泽型COC，选择476个用于IVM，导致270个（56.7%）受精卵分裂，产生137个囊胚（28.8%）。河流型和F1胚胎在体外发育的第6至7天（对应于囊胚扩张期）分别移植到经Ovsynch同步化处理的F1受体和自然发情后的沼泽型水牛受体中，每个受体移植两个胚胎。根据胚胎移植时经直肠触诊卵巢的情况，47个经Ovsynch同步化处理的F1受体中有26个（55.3%）被认为适合移植，由于妊娠2至3个月期间发生了3次流产，导致7次妊娠（26.9%）和4次产犊（15.3%）。总共29个自然发情后的沼泽型受体被判定适合作为受体，由于妊娠2个月和3个月时分别发生了2次流产，导致12次妊娠（41.4%）和10次产犊（34.5%）。河流型和F1胚胎移植后的妊娠率和产犊率相似。同样，来自河流型和F1胚胎移植的犊牛出生体重也没有差异：分别为30.5±1.4和32.9±2.4。本研究中作为参考的一组河流型和沼泽型水牛人工授精后的妊娠率和产犊率与移植体外生产胚胎的受体相似。总体而言，所产活犊未记录到明显的产后并发症。
