Yasuda Kazunori, Sakai Toshihiko, Kondo Eiji, Onodera Shin
Department of Sports Medicine and Joint Reconstruction Surgery, Hokkaido University School of Medicine, Kita-15 Nishi-7, Kita-ku, Sapporo 060-8638, Japan.
Clin Biomech (Bristol). 2007 Oct;22(8):941-9. doi: 10.1016/j.clinbiomech.2007.05.007. Epub 2007 Jun 29.
The purpose of this study is to biomechanically and histologically evaluate the effect of bleeding from bone marrow on remodeling of the in situ frozen-thawed anterior cruciate ligament.
Forty-four rabbits were used. Eight rabbits were used to evaluate the amount of bleeding at days 1-4. Thirty-six rabbits were divided into two groups, after the right anterior cruciate ligament underwent the freeze-thaw treatment. In Group I, no treatments were applied. In Group II, a tunnel was drilled into the bone marrow the femoral intercondylar notch. Each rabbit was sacrificed at 6 or 12 weeks. We examined the mechanical properties, and the histology of the anterior cruciate ligament.
A small amount of blood clot was observed only on the first day in Group I, while a large amount of blood clot was seen around the anterior cruciate ligament for 3 days after surgery in Group II. In the midsubstance, a number of cells were scattered in Group II, no cells were seen in Group I at 6 weeks. The tangent modulus showed some tendency of difference between Groups I and II at each period.
Bleeding from the bone marrow obviously enhanced extrinsic cell infiltration into the in situ frozen-thawed anterior cruciate ligament at 6 weeks, and showed some effects on its mechanical properties. This study implied that, in anterior cruciate ligament reconstruction, the blood from a bone tunnel play an important role in graft remodeling.
本研究旨在从生物力学和组织学方面评估骨髓出血对原位冻融前交叉韧带重塑的影响。
使用了44只兔子。8只兔子用于评估第1 - 4天的出血量。36只兔子在右侧前交叉韧带进行冻融处理后分为两组。第一组不进行任何处理。第二组在股骨髁间切迹的骨髓中钻一个隧道。每只兔子在6周或12周时处死。我们检查了前交叉韧带的力学性能和组织学情况。
第一组仅在第一天观察到少量血凝块，而第二组在术后3天在前交叉韧带周围可见大量血凝块。在韧带中部，第二组在6周时有许多细胞散在分布，第一组未见细胞。在每个时期，两组之间的切线模量显示出一定的差异趋势。
骨髓出血在6周时明显增强了外在细胞向原位冻融前交叉韧带内的浸润，并对其力学性能产生了一定影响。本研究表明，在前交叉韧带重建中，骨隧道中的血液在移植物重塑中起重要作用。
