Jain Deepak, Ebine Naoyuki, Jia Xiaoming, Kassis Amira, Marinangeli Christopher, Fortin Marc, Beech Robin, Hicks Kevin B, Moreau Robert A, Kubow Stan, Jones Peter J H
School of Dietetics and Human Nutrition, Macdonald Campus of McGill University, Ste-Anne-de-Bellevue, QC, Canada.
J Nutr Biochem. 2008 Apr;19(4):229-36. doi: 10.1016/j.jnutbio.2007.02.012. Epub 2007 Jun 29.
The aim of this study was to investigate the cholesterol-lowering mechanisms of corn fiber oil (CFO), ferulate phytostanyl esters (FPEs) and parent compounds of FPE, including sitostanol and ferulic acid, in hamsters.
Seventy male Golden Syrian hamsters were randomly assigned to six experimental diets for 4 weeks: (1) cornstarch-casein-sucrose-based control diet (control); and (2) control diet plus 0.1% (wt/wt) cholesterol (cholesterol-control). The remaining four groups were given cholesterol-control diet with: (3) 10% (wt/wt) CFO; (4) 0.5% (wt/wt) sitostanol; (5) 0.23% (wt/wt) ferulic acid; and (6) 0.73% (wt/wt) FPE. At the end of dietary intervention, total plasma cholesterol, high-density lipoprotein cholesterol and triglyceride concentrations were determined. Parameters of cholesterol kinetics, including cholesterol absorption and synthesis, as well as mRNA expression of sterol transporters such as Niemann-Pick C1 like 1 (NPC1L1), ATP-binding cassette G5 (ABCG5) and ABCG8, were assessed.
Supplementation with CFO decreased (P<.0001) plasma total cholesterol levels by 29% as compared with the cholesterol-control group, while FPE and sitostanol reduced (P<.02) cholesterolemia by 15% and 14%, respectively. CFO and sitostanol decreased (P<.05) cholesterol absorption by 24% compared to the cholesterol-control group. Dietary intervention did not alter the intestinal gene expression of ABCG5, ABCG8 and NPC1L1.
The present results show that the CFO-induced and sitostanol-induced decrease in cholesterol absorption is independent of intestinal enterocyte sterol transporters such as ABCG5, ABCG8 and NPC1L1 in hamsters.
本研究旨在探讨玉米纤维油(CFO)、阿魏酸植物甾烷醇酯(FPEs)及其母体化合物(包括谷甾烷醇和阿魏酸)对仓鼠的降胆固醇机制。
将70只雄性叙利亚金黄地鼠随机分为六组,分别给予六种实验性饮食,为期4周:(1)以玉米淀粉-酪蛋白-蔗糖为基础的对照饮食(对照组);(2)对照饮食加0.1%(重量/重量)胆固醇(胆固醇对照组)。其余四组给予胆固醇对照组饮食,并分别添加:(3)10%(重量/重量)CFO;(4)0.5%(重量/重量)谷甾烷醇;(5)0.23%(重量/重量)阿魏酸;(6)0.73%(重量/重量)FPE。在饮食干预结束时,测定血浆总胆固醇、高密度脂蛋白胆固醇和甘油三酯浓度。评估胆固醇动力学参数,包括胆固醇吸收和合成,以及固醇转运蛋白如尼曼-匹克C1样1(NPC1L1)、ATP结合盒转运体G5(ABCG5)和ABCG8的mRNA表达。
与胆固醇对照组相比,补充CFO可使血浆总胆固醇水平降低29%(P<0.0001),而FPE和谷甾烷醇分别使胆固醇血症降低15%和14%(P<0.02)。与胆固醇对照组相比,CFO和谷甾烷醇可使胆固醇吸收降低24%(P<0.05)。饮食干预未改变ABCG5、ABCG8和NPC1L1的肠道基因表达。
目前的结果表明,在仓鼠中,CFO和谷甾烷醇诱导的胆固醇吸收降低与肠道肠上皮细胞固醇转运蛋白如ABCG5、ABCG8和NPC1L1无关。