Tsukagoshi N, Petersen M H, Huber U, Franklin R M, Seelig J
Eur J Biochem. 1976 Feb 16;62(2):257-62. doi: 10.1111/j.1432-1033.1976.tb10155.x.
An unsaturated fatty acid auxotroph, strain UFA, isolated from the marine pseudomonad Pseudomonas BAL-31, host cell of the lipid-containing bacteriophage PM2, was grown in media supplemented with different unsaturated fatty acids. Under these conditions the fatty acid composition of the cell could be altered drastically. The phase transition in the native membrane and in the extracted lipids was analyzed by electron spin resonance using a nitroxide spin probe. Membranes prepared from strain UFA grown in cis16:1 or trans16:1 showed one transition at 9.4 degrees C and 12.4 degrees C respectively. Extracted lipids in both cases had almost the same transition temperature as that of the intact membrane. Membranes prepared from Pseudomonas BAL-31 had one transition at approximately 12 degrees C, on the other hand there was no clear cut phase transition using extracted lipids. Replication of bacteriophage PM2 took place below the transition temperature of the membrane lipids in the case where strain UFA was grown in tran16:1. Other cases were not studied.
从含脂质噬菌体PM2的宿主细胞——海洋假单胞菌假单胞菌BAL - 31中分离出的不饱和脂肪酸营养缺陷型菌株UFA，在添加了不同不饱和脂肪酸的培养基中生长。在这些条件下，细胞的脂肪酸组成会发生显著变化。使用氮氧化物自旋探针通过电子自旋共振分析天然膜和提取脂质中的相变。由在顺式16:1或反式16:1中生长的UFA菌株制备的膜分别在9.4℃和12.4℃出现一个转变。在这两种情况下，提取的脂质具有与完整膜几乎相同的转变温度。另一方面，由假单胞菌BAL - 31制备的膜在约12℃出现一个转变，而使用提取的脂质则没有明显的相变。在菌株UFA在反式16:1中生长的情况下，噬菌体PM2的复制发生在膜脂质的转变温度以下。其他情况未作研究。
