Ksiaa Leila, Ayed-Jendoubi Saloua, Sfar Imen, Gorgi Yousr, Najjar Houda Aouadi Tawfik, Abdallah Taieb Ben, Ayed Khaled
Immunology Laboratory, Charles Nicolle Hospital, Tunis, Tunisia.
Viral Immunol. 2007 Summer;20(2):312-9. doi: 10.1089/vim.2006.0060.
Human leukocyte antigens (HLAs) of class I and class II are reported to influence the outcome of hepatitis C virus (HCV) infection. The aim of this study was to assess the role of HLA class I and class II in influencing spontaneous viral clearance or persistence in HCV-infected patients. HLA class I (A and B) typing was performed by lymphocytotoxicity test and HLA class II (DRB1) was determined by low-resolution PCR-SSP (polymerase chain reaction amplification with sequence-specific primers) for 99 subjects (48 men and 51 women). Of these, 75 had chronic infection and 24 had viral clearance. No significant differences were observed between individuals with spontaneous viral clearance or chronic HCV infection for age, sex, source of infection, and risk factors. HLAB-w35 and HLA-DRB108 occurred more frequently in those with viral clearance (21.7 and 16.6%, respectively) compared with those with chronic infection (5.5 and 2.6%; p < 0.04 and p < 0.01, respectively). DRB115 occurred more often in those with chronic infection (29.3%) compared with those with viral clearance (16.66%), but the difference did not reach statistical significance. These results support the hypothesis that specific HLA class I and class II alleles might influence the clearance or persistence of HCV infection. Both Bw35 and DRB108 are associated with clearance of circulating HCV whereas DRB115 appears to predispose to progression of liver disease in Tunisian patients. Taken together, our results and those previously reported suggest that HLA associations with the outcome of hepatitis C viremia vary in relation to the ethnicity of the population studied. Further prospective studies of larger cohorts of HCV-infected subjects are needed to evaluate, in different populations, the role of specific HLA class I and class II alleles in the outcome of HCV infection.
据报道，I类和II类人类白细胞抗原（HLA）会影响丙型肝炎病毒（HCV）感染的结果。本研究的目的是评估I类和II类HLA在影响HCV感染患者病毒自发清除或持续存在方面的作用。对99名受试者（48名男性和51名女性）进行淋巴细胞毒性试验进行I类HLA（A和B）分型，通过低分辨率PCR-SSP（序列特异性引物聚合酶链反应扩增）确定II类HLA（DRB1）。其中，75例为慢性感染，24例病毒清除。在病毒自发清除或慢性HCV感染个体之间，年龄、性别、感染源和危险因素方面未观察到显著差异。与慢性感染个体（分别为5.5%和2.6%；p<0.04和p<0.01）相比，病毒清除个体中HLAB-w35和HLA-DRB108出现频率更高（分别为21.7%和16.6%）。与病毒清除个体（16.66%）相比，慢性感染个体中DRB115出现频率更高（29.3%），但差异未达到统计学意义。这些结果支持以下假设：特定的I类和II类HLA等位基因可能影响HCV感染的清除或持续存在。Bw35和DRB108均与循环HCV的清除相关，而DRB115似乎易导致突尼斯患者肝病进展。综上所述，我们的结果和先前报道的结果表明，HLA与丙型肝炎病毒血症结果的关联因所研究人群的种族而异。需要对更大队列的HCV感染受试者进行进一步的前瞻性研究，以评估不同人群中特定的I类和II类HLA等位基因在HCV感染结果中的作用。
