Kamel Ayman H
Department of Chemistry, Faculty of Science, Ain-Shams University, Cairo, Egypt.
J Pharm Biomed Anal. 2007 Oct 18;45(2):341-8. doi: 10.1016/j.jpba.2007.05.012. Epub 2007 May 18.
The potentiometric response properties of several PVC-based membrane sensors using phthalocyanine complexes of cobalt(II) (CoPC) and Fe(II) (FePC) as anion carriers, towards uric acid were constructed and characterized. The sensors demonstrated fast near-Nernstian response for uric acid over the concentration ranges 9.1 x 10(-6) to 9.1 x 10(-2) and 3.1 x 10(-5) to 3.1 x 10(-2)M with detection limits 0.67 and 2.85 microg mL(-1) over pH 6.5-8 for CoPC and FePC based membrane sensors plasticized with o-NPOE and 1% TDMAC, respectively. A novel solid-state planar chip urate sensor was developed, characterized according to IUPAC recommendations, easily used in a single channel wall-jet flow injection system and compared with a tubular detector. The intrinsic characteristics of the detectors in a low dispersion manifold were determined and compared with data obtained under hydrodynamic mode of operation. Validation of the assay methods with the proposed sensors by measuring the lower limit, range, accuracy, precision, repeatability and between-day-variability revealed good performance characteristics confirming applicability for continuous determination of uric acid. The sensors were used for determining urate in biological fluids at an input rate of 50 samples per hour. The results compare favorably with data obtained by the standard spectrophotometry.
构建并表征了几种以钴（II）（CoPC）和铁（II）（FePC）的酞菁配合物作为阴离子载体的基于聚氯乙烯（PVC）的膜传感器对尿酸的电位响应特性。对于分别用邻硝基苯辛醚（o-NPOE）和1%十四烷基三甲基氯化铵（TDMAC）增塑的基于CoPC和FePC的膜传感器，在9.1×10⁻⁶至9.1×10⁻²以及3.1×10⁻⁵至3.1×10⁻²M的尿酸浓度范围内，传感器表现出快速的近能斯特响应，在pH 6.5 - 8时检测限分别为0.67和2.85 μg mL⁻¹。开发了一种新型固态平面芯片尿酸盐传感器，根据国际纯粹与应用化学联合会（IUPAC）的建议进行了表征，该传感器易于在单通道壁流喷射流动注射系统中使用，并与管式检测器进行了比较。测定了低分散歧管中检测器的固有特性，并与在流体动力学操作模式下获得的数据进行了比较。通过测量下限、范围、准确度、精密度、重复性和日间变异性对所提出的传感器的分析方法进行验证，结果显示出良好的性能特征，证实了其适用于尿酸的连续测定。这些传感器用于以每小时50个样品的输入速率测定生物流体中的尿酸盐。结果与通过标准分光光度法获得的数据相比具有优势。
