Hashizume Tomomi, Momoi Fumiki, Kurita-Ochiai Tomoko, Kaminogawa Shuichi, Hosono Akira, Kataoka Kosuke, Shinozaki-Kuwahara Noriko, Kweon Mi-Na, Yamamoto Masafumi
Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, 2-870-1, Sakaecho-Nishi, Matsudo, Chiba 271-8587, Japan.
Biochem Biophys Res Commun. 2007 Aug 24;360(2):388-93. doi: 10.1016/j.bbrc.2007.06.096. Epub 2007 Jun 26.
In this study, we investigated whether isolated lymphoid follicles (ILF) play a role in the regulation of intestinal IgA antibody (Ab) responses. The transfer of wild type (WT) bone marrow (BM) to lymphotoxin-alpha-deficient (LTalpha(-/-)) mice resulted in the formation of mature ILF containing T cells, B cells, and FDC clusters in the absence of mesenteric lymph nodes and Peyer's patches. Although the ILF restored total IgA Abs in the intestine, antigen (Ag)-specific IgA responses were not induced after oral immunization with recombinant Salmonella expressing fragment C of tetanus toxin. Moreover, Ag-specific cell proliferation was not detected in the ILF. Interestingly, no IgA anti-LPS Abs were detected in the fecal extracts of LTalpha(-/-) mice reconstituted with WT BM. On the basis of these findings, ILF can be presumed to play a role in the production of IgA Abs, but lymphoid nodules are not inductive sites for the regulation of Ag-specific intestinal IgA responses to recombinant Salmonella.
在本研究中，我们调查了孤立淋巴滤泡（ILF）是否在肠道IgA抗体（Ab）反应的调节中发挥作用。将野生型（WT）骨髓（BM）移植到淋巴毒素-α缺陷（LTα(-/-)）小鼠体内，导致在没有肠系膜淋巴结和派尔集合淋巴结的情况下形成了含有T细胞、B细胞和滤泡树突状细胞簇的成熟ILF。尽管ILF恢复了肠道中的总IgA抗体，但在用表达破伤风毒素片段C的重组沙门氏菌进行口服免疫后，并未诱导出抗原（Ag）特异性IgA反应。此外，在ILF中未检测到Ag特异性细胞增殖。有趣的是，在用WT BM重建的LTα(-/-)小鼠的粪便提取物中未检测到IgA抗LPS抗体。基于这些发现，可以推测ILF在IgA抗体的产生中发挥作用，但淋巴小结并非调节对重组沙门氏菌的Ag特异性肠道IgA反应的诱导部位。
