Murai R, Kanbe T, Mukoyama T, Shimomura T, Hashiguchi K, Yoshida Y, Tsuchiya H, Hoshikawa Y, Kurimasa A, Shiota G
Division of Molecular and Genetic Medicine, Department of Genetic Medicine and Regenerative Therapeutics, Graduate School of Medicine, Tottori University, Yonago 683-8504, Japan.
Inflamm Res. 2007 Jun;56(6):240-5. doi: 10.1007/s00011-007-6100-z.
Since rebamipide is effective for the treatment of ulcerative colitis (UC), we examined the involvement of hepatocyte growth factor (HGF) in the action of rebamipide.
Fifty-five and forty female Balb/c mice, respectively, were used in Exp. 1 and 2.
50 mg/kg/day rebamipide (Exp. 1) and 1 x 10(7) pfu pAxCAHGF (the CAG promoter-driving HGF gene in adenovirus vector) (Exp. 2) were intrarectally introduced after induction of colitis by 4 % dextran sulfate sodium (DSS).
Therapeutic effects were assessed by cell proliferation and apoptosis.
Rebamipide caused proliferation of epithelial cells at 10 days after treatment, and decreased apoptosis at 10, 14 and 21 days, compared with controls. Expression of HGF was greatly increased in rebamipide-treated mice. pAxCAHGF caused cell proliferation and apoptosis, which showed the same pattern as with rebamipide treatment.
Rectal administration of rebamipide is effective for DSS-induced colitis in association with induction of HGF.
由于瑞巴派特对溃疡性结肠炎（UC）的治疗有效，我们研究了肝细胞生长因子（HGF）在瑞巴派特作用中的参与情况。
实验1和实验2分别使用了55只和40只雌性Balb/c小鼠。
在通过4%硫酸葡聚糖钠（DSS）诱导结肠炎后，经直肠给予50mg/kg/天的瑞巴派特（实验1）和1×10⁷ pfu pAxCAHGF（腺病毒载体中CAG启动子驱动的HGF基因）（实验2）。
通过细胞增殖和凋亡评估治疗效果。
与对照组相比，瑞巴派特在治疗后10天引起上皮细胞增殖，并在10、14和21天减少凋亡。瑞巴派特治疗的小鼠中HGF的表达显著增加。pAxCAHGF引起细胞增殖和凋亡，其模式与瑞巴派特治疗相同。
经直肠给予瑞巴派特对DSS诱导的结肠炎有效，且与HGF的诱导有关。
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