Iwai A, Iwashita E
Second Department of Internal Medicine, National Defense Medical College, School of Medicine, Tokorozawa Saitama, Japan.
Dig Dis Sci. 1998 Sep;43(9 Suppl):143S-147S.
Earlier studies have shown the antiinflammatory effects of histamine and nitric oxide (NO) in a model of colitis induced by DSS. However, the defense system against free radicals in this model remained unclear. The aim of this study was to evaluate the effects of rebamipide, which inhibits the production of free radicals, in this model using male Sprague-Dawley rats. Colitis induced by 1% DSS is characterized by slow, weak inflammation and is regarded as a chronic inflammation model. In contrast, colitis induced by 4% DSS is characterized by fast, strong inflammation and is regarded as the acute inflammation model. Endoscopic examinations, peripheral white blood cell (WBC) counts, and assays of myeloperoxidase activity (MPO) in homogenates of colon mucosa were performed after one week (4% DSS model) and eight weeks (1% DSS model). Inflammation of colon mucosa was milder in the rats given rebamipide compared with controls in both the 4% and 1% DSS model. Furthermore, peripheral WBC counts correlated with colonic MPO activity. These findings indicate that rebamipide works as an antiinflammatory agent in both acute and chronic inflammation.
早期研究已表明组胺和一氧化氮（NO）在葡聚糖硫酸钠（DSS）诱导的结肠炎模型中具有抗炎作用。然而，该模型中针对自由基的防御系统仍不清楚。本研究的目的是使用雄性Sprague-Dawley大鼠，评估抑制自由基产生的瑞巴派特在该模型中的作用。1% DSS诱导的结肠炎表现为炎症缓慢、轻微，被视为慢性炎症模型。相比之下，4% DSS诱导的结肠炎表现为炎症快速、强烈，被视为急性炎症模型。在一周后（4% DSS模型）和八周后（1% DSS模型）进行了内镜检查、外周白细胞（WBC）计数以及结肠黏膜匀浆中髓过氧化物酶活性（MPO）测定。在4%和1% DSS模型中，给予瑞巴派特的大鼠结肠黏膜炎症均比对照组轻。此外，外周WBC计数与结肠MPO活性相关。这些发现表明瑞巴派特在急性和慢性炎症中均发挥抗炎作用。
