指示性检测发现两种未经批准的转基因水稻品系污染了粉丝产品。

Indicated detection of two unapproved transgenic rice lines contaminating vermicelli products.

作者信息

Akiyama Hiroshi, Sasaki Nobuhiro, Sakata Kozue, Ohmori Kiyomi, Toyota Akie, Kikuchi Yutaka, Watanabe Takahiro, Furui Satoshi, Kitta Kazumi, Maitani Tamio

机构信息

National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo 184-8588, Japan.

出版信息

J Agric Food Chem. 2007 Jul 25;55(15):5942-7. doi: 10.1021/jf070508m. Epub 2007 Jul 4.

DOI:10.1021/jf070508m

PMID:17608495

Abstract

We analyzed the DNA fragments extracted from four rice vermicelli products. The Bacillus thuringiensis (Bt) rice line, which has a construct similar to the GM Shanyou 63 line, was detected in some vermicelli products by identification of the junction region sequence between rice Act1 promoter and the Cry1Ac gene, and that between Cry1Ac and nos. In addition, we also detected a different Bt rice line by means of the junction region sequence between the maize ubiquitin promoter and cry1Ab gene and that between the cauliflower mosaic virus 35S promoter and the hygromycin phosphotransferase in some vermicelli products. Accordingly, we for the first time have detected the two transgenic Bt rice lines contaminating rice vermicelli samples. Furthermore, we developed a duplex real-time polymerase chain reaction (PCR) method for the simultaneous detection of both Bt rice lines.

摘要

我们分析了从四种米粉产品中提取的DNA片段。通过鉴定水稻Act1启动子与Cry1Ac基因之间以及Cry1Ac与nos之间的连接区序列，在一些米粉产品中检测到了与转基因汕优63品系构建体相似的苏云金芽孢杆菌（Bt）水稻品系。此外，我们还通过玉米泛素启动子与cry1Ab基因之间以及花椰菜花叶病毒35S启动子与潮霉素磷酸转移酶之间的连接区序列，在一些米粉产品中检测到了另一种Bt水稻品系。因此，我们首次检测到两种转基因Bt水稻品系污染米粉样品。此外，我们开发了一种双重实时聚合酶链反应（PCR）方法，用于同时检测这两种Bt水稻品系。