Li Yijin, Ma Lianli, Shen Jikun, Chong Anita S
Department of Surgery, Section of Transplantation, University of Chicago, Chicago, IL 60637, USA.
Proc Natl Acad Sci U S A. 2007 Jul 17;104(29):12093-8. doi: 10.1073/pnas.0705240104. Epub 2007 Jul 3.
Alloreactive B cells can contribute to graft rejection. Anti-CD154 treatment together with donor-specific transfusion (DST) results in the long-term survival of MHC-mismatched mouse heart grafts and inhibition of alloantibody production. To characterize the mechanism of B cell tolerance induced by the anti-CD154 and DST, we used 3-83Igi mice, on BALB/c (H-2K(d)) background, that express a B cell receptor that reacts with MHC class I antigens H-2K(b). Transplanting C57BL/6 (H-2K(b)) hearts into 3-83Igi mice, followed by tolerance induction, resulted in the peripheral deletion of mature but not immature 3-83 B cells. The sustained deletion of mature alloreactive B cells required the presence of the allograft and can be explained by the absence of T cell help.
同种反应性B细胞可导致移植物排斥反应。抗CD154治疗联合供体特异性输血（DST）可使MHC不匹配的小鼠心脏移植物长期存活，并抑制同种抗体产生。为了阐明抗CD154和DST诱导B细胞耐受的机制，我们使用了背景为BALB/c（H-2K(d)）的3-83Igi小鼠，这些小鼠表达与MHC I类抗原H-2K(b)反应的B细胞受体。将C57BL/6（H-2K(b)）心脏移植到3-83Igi小鼠体内，随后诱导耐受，导致成熟而非未成熟的3-83 B细胞在外周缺失。成熟同种反应性B细胞的持续缺失需要同种异体移植物的存在，这可以用缺乏T细胞辅助来解释。
