Filipović Branko, Sosić-Jurjević Branka, Ajdzanović Vladimir, Trifunović Svetlana, Manojlović-Stojanoski Milica, Ristić Natasa, Nestorović Natasa, Milosević Verica, Sekulić Milka
Institute for Biological Research, Blv. Despota Stefana 142, 11060, Belgrade, Serbia.
Histochem Cell Biol. 2007 Aug;128(2):153-9. doi: 10.1007/s00418-007-0307-5. Epub 2007 Jul 3.
This study was to evaluate the effect of androgen deficiency on thyroid immunoreactive C-cells and bone structure and function in a male orchidectomized middle-aged rat model. Fifteen-month-old male Wistar rats were divided into orchidectomized (Orx) and the sham-operated control (Sham) group. In the Orx group significant decreases (P < 0.05) were found in the volume of C cells (by 14%), their relative volume density (by 13%) and serum calcitonin concentration (by 54%) compared to the controls. Analyses of trabecular microarchitecture of the proximal tibia metaphysis showed that Orx induced marked decreases of cancellous bone area, trabecular thickness and trabecular number (by 52, 20 and 19% respectively; P < 0.05), whereas trabecular separation was increased by 27% (P < 0.05). In Orx rats, serum osteocalcin concentration was increased by 119% (P < 0.05), while serum calcium and phosphorus were 6 and 14% (P < 0.05) lower, respectively, compared to the levels in the Sham. In addition, urine calcium content was considerably higher (by 129%; P < 0.05) in Orx animals. These findings indicate that the androgen deficiency caused by Orx in middle-aged rats modulated the structure of C cells and diminished secretion of calcitonin. Histomorphometrical and biochemical analyses demonstrated a decrease of cancellous bone mass and increased bone turnover.
本研究旨在评估雄激素缺乏对雄性去势中年大鼠模型中甲状腺免疫反应性C细胞以及骨结构和功能的影响。将15月龄雄性Wistar大鼠分为去势（Orx）组和假手术对照组（Sham）。与对照组相比，Orx组C细胞体积（减少14%）、相对体积密度（减少13%）和血清降钙素浓度（减少54%）均显著降低（P<0.05）。胫骨近端干骺端小梁微结构分析显示，Orx导致松质骨面积、小梁厚度和小梁数量显著减少（分别减少52%、20%和19%；P<0.05），而小梁间距增加27%（P<0.05）。与Sham组相比，Orx大鼠血清骨钙素浓度增加119%（P<0.05），而血清钙和磷水平分别降低6%和14%（P<0.05）。此外，Orx动物的尿钙含量显著更高（增加129%；P<0.05）。这些发现表明，中年大鼠Orx所致的雄激素缺乏调节了C细胞结构并减少了降钙素分泌。组织形态计量学和生化分析表明松质骨量减少且骨转换增加。
