一家三级护理医院中用于诊断肺炎球菌性和脑膜炎球菌性脑膜炎的实时聚合酶链反应检测方法的评估
Evaluation of a real-time polymerase chain reaction assay for the diagnosis of pneumococcal and meningococcal meningitis in a tertiary care hospital.
作者信息
Van Gastel E, Bruynseels P, Verstrepen W, Mertens A
机构信息
Department of Microbiology, ZNA Middelheim, Antwerp, Belgium.
出版信息
Eur J Clin Microbiol Infect Dis. 2007 Sep;26(9):651-3. doi: 10.1007/s10096-007-0350-0.
This study evaluated the performance of a real-time polymerase chain reaction (PCR) assay in comparison with Gram staining and culture of cerebrospinal fluid for the diagnosis of meningococcal and pneumococcal meningitis in patients with suspected bacterial meningitis. The sensitivity for detection of Neisseria meningitidis in cerebrospinal fluid was 87% (20/23) for the PCR assay, 27% (6/22) for Gram staining, and 17% (4/23) for culture. The sensitivity for detection of Streptococcus pneumoniae in cerebrospinal fluid was 100% (14/14) for the PCR assay, 62% (8/13) for Gram staining, and 36% (5/14) for culture. Therefore, we recommend that real-time PCR of cerebrospinal fluid for detection of N. meningitidis and S. pneumoniae become a part of the routine diagnostic procedure for patients with suspected bacterial meningitis.
本研究评估了实时聚合酶链反应（PCR）检测法与脑脊液革兰氏染色及培养法相比，在诊断疑似细菌性脑膜炎患者的脑膜炎球菌性和肺炎球菌性脑膜炎中的表现。脑脊液中检测脑膜炎奈瑟菌的灵敏度，PCR检测法为87%（20/23），革兰氏染色法为27%（6/22），培养法为17%（4/23）。脑脊液中检测肺炎链球菌的灵敏度，PCR检测法为100%（14/14），革兰氏染色法为62%（8/13），培养法为36%（5/14）。因此，我们建议，对疑似细菌性脑膜炎患者，脑脊液实时PCR检测脑膜炎奈瑟菌和肺炎链球菌应成为常规诊断程序的一部分。
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