Velivetskaya Tatiana A, Ignatyev Alexander V, Reize Marina V, Kiyashko Serguei I
Far East Geological Institute FEB RAS, Vladivostok 690022, Russia.
Rapid Commun Mass Spectrom. 2007;21(15):2451-5. doi: 10.1002/rcm.3112.
A simple and effective method for the conversion of organic carbon into carbon dioxide for analysis of stable carbon isotopes (delta(13)C) in samples of various organic substances, soils, sedimentary rocks, oils and volatile organic liquids is presented. The conversion of organic carbon of the samples is carried out in a quartz reactor connected to a vacuum line for CO(2) freezing and purification. A solid organic sample mixed with CuO is placed at the reactor bottom and the reactor is subsequently filled with granular CuO. One end of the CuO column is preheated to 850 degrees C while the other end of the column in contact with the sample is kept at ambient temperature. Heating of the sample (850 degrees C) and the remainder of the column is then performed. The preheated part of the column provides efficient conversion of carbon into CO(2). The reactor for the conversion of volatile liquid organic compounds is filled with granular CuO. The column of CuO is heated to 850 degrees C. Samples of volatile liquids are introduced into the reactor through a septum using a microsyringe. Complete conversion takes 10 min for solid samples and 3 min for volatile liquids. The precision of the delta(13)C analysis for solid and volatile liquid organic substances is +/-0.1 per thousand and +/-0.04 per thousand, respectively.
本文介绍了一种简单有效的方法，可将有机碳转化为二氧化碳，用于分析各种有机物质、土壤、沉积岩、石油和挥发性有机液体样品中的稳定碳同位素（δ(13)C）。样品中有机碳的转化在连接到用于二氧化碳冷冻和纯化的真空管路的石英反应器中进行。将与氧化铜混合的固体有机样品置于反应器底部，随后在反应器中填充粒状氧化铜。氧化铜柱的一端预热至850℃，而与样品接触的柱的另一端保持在环境温度。然后对样品（850℃）和柱的其余部分进行加热。柱的预热部分可实现碳向二氧化碳的高效转化。用于挥发性液体有机化合物转化的反应器中填充有粒状氧化铜。氧化铜柱加热至850℃。使用微量注射器通过隔膜将挥发性液体样品引入反应器。固体样品完全转化需10分钟，挥发性液体需3分钟。固体和挥发性液体有机物质的δ(13)C分析精度分别为±0.1‰和±0.04‰。
