Yang Cheng, Li Guan-cheng, Li Yue-hui, Hu Jin-yue, Xiao Yan, Zhang Zhi-jie
Cancer Research Institute of Central South University,Changsha 410078, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Jun;32(3):380-6.
To investigate the biological properties of Caski cell lines induced by exposing to the space environment.
Caski cells were carried in "Shen Zhou IV" airship. After 7 days of spaceflight, cells survived and were monocoloned, and the experimental methods such as cell morphological observation, the cell proliferation assay, flow cytometry cell cycle analysis, the soft agar assay, and tumorigenesis assay were used to analyze cell growth characteristics and malignant phenotypes.
Altogether 1440 strains subclonal cell lines were established and 4 strains were screened. Compared with the control group, mutated cells appeared to have multiple cell morphological changes. Strains numbered 44F10 and 17E3 were screened due to their increased cell proliferation and tumorigenesis, and their cell cycles were induced to progress from G(1) to S phase, while strains 48A9 and 31F2 were opposite to 44F10 and 17E3 in cytological events. The average population double time of ground nomal control group, ground simulant control group, strains numbered 44F10, 17E3, 48A9 and 31F2 groups were 56.54, 58.44, 52.96, 51.46, 101.76 and 88.47h, respectively; compared with the control group, the average double time of strains numbered 44F10 and 17E3 was decline, but with no statistical significance. However, compared with the control groups, the average double time of 48A9 and 31F2 was significant increased (P<0.05). The colony formation rates were 9.7%, 9.3%, 14.7%, 12.1%, 0 and 0.1%, respectively, and the difference between the ground control groups and the other groups was significant (P<0.01); 6 groups of above-mentioned Caski cells were inoculated subcutaneously in Babl/c nude mice respectively. Forty-seven days later, the formed tumors in the nude mice were statistically analyzed and tested. The average weight of tumors of the above-mentioned groups was 0.066, 0.066, 0.175, 0.249, 0.011 and 0.018g. The difference between the ground control groups and other groups was significant (P<0.05).
Spaceflight may affect the physiological characteristics of tumor cells and the variation is complicated.
研究经空间环境暴露诱导的Caski细胞系的生物学特性。
将Caski细胞搭载于“神舟四号”飞船。经过7天的太空飞行后，存活的细胞进行单克隆培养，并采用细胞形态学观察、细胞增殖检测、流式细胞术细胞周期分析、软琼脂检测及肿瘤形成检测等实验方法分析细胞生长特性及恶性表型。
共建立了1440个亚克隆细胞系，筛选出4个细胞系。与对照组相比，突变细胞出现多种细胞形态变化。筛选出44F10和17E3号细胞系，因其细胞增殖和肿瘤形成能力增强，且细胞周期被诱导从G(1)期进入S期，而48A9和31F2号细胞系在细胞学事件上与44F10和17E3号细胞系相反。地面对照组、地面模拟对照组、44F10、17E3、48A9和31F2号细胞系组的平均群体倍增时间分别为56.54、58.44、52.96、51.46、101.76和88.47小时；与对照组相比，44F10和17E3号细胞系的平均倍增时间缩短，但无统计学意义。然而，与对照组相比，48A9和31F2号细胞系的平均倍增时间显著延长（P<0.05）。集落形成率分别为9.7%、9.3%、14.7%、12.1%、0和0.1%，地面对照组与其他组之间差异有统计学意义（P<0.01）；将上述6组Caski细胞分别皮下接种于Babl/c裸鼠。47天后，对裸鼠体内形成的肿瘤进行统计学分析和检测。上述各组肿瘤的平均重量分别为0.066、0.066、0.175、0.249、0.011和0.018克。地面对照组与其他组之间差异有统计学意义（P<0.05）。
太空飞行可能影响肿瘤细胞的生理特性，且变异情况复杂。
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