Jiang Jie, Xia Min, Feng Jin-Bo, Kong Bei-Hua
Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan 250012, China.
Zhonghua Fu Chan Ke Za Zhi. 2007 Jun;42(6):398-402.
To study the inhibition effects of breast cancer metastasis suppressor l (BRMS1) gene on metastasis of human ovarian cancer cell in vivo and in vitro.
BRMS1 gene was transfected into human ovarian cancer cell line A2780 by liposome transfection method. The cells were divided into 3 groups: transfected group with pcDNA3-BRMS1, negative control group with empty plasmid pcDNA3, blank control group without any transfection. Proliferation, apoptosis, growth rate, capability of colony formation, gap junctional intercellular communication (GJIC), capability of adhesion, changes of surface and internal structures of cells were observed in vitro. The cells were injected into athymic mice to establish animal tumor models, and inhibition of the tumorigenicity and metastasis were observed in vivo.
BRMS1 gene was transfected into A2780 cell line successfully. There were no significant differences in the growth rate among transfected group, negative control group and blank control group (P > 0.05). The amounts of cell clones per well were 40 +/- 4 in transfected group, 42 +/- 7 in blank control group and 39 +/- 4 in negative control group (P > 0.05 between each two groups). The ratios of S vs G(2)/M and G(0)/G(1) were not significantly different in three groups (P > 0.05). The ultramicrostructure of cells detected by electron microscope showed that GJIC function in transfected group was higher than that in the other two groups. While in migration assay, the numbers of cells in lower chamber passing through the membrane in transfected group, blank control group and negative control group were 112 +/- 23, 306 +/- 49 and 322 +/- 91, respectively; with significant differences among 3 groups (P < 0.01).
BRMS1 gene could suppress metastasis of ovarian cancer in vitro and in vivo.
研究乳腺癌转移抑制基因1（BRMS1）对人卵巢癌细胞体内外转移的抑制作用。
采用脂质体转染法将BRMS1基因转染至人卵巢癌细胞系A2780。细胞分为3组：pcDNA3-BRMS1转染组、空质粒pcDNA3阴性对照组、未转染空白对照组。体外观察细胞的增殖、凋亡、生长速率、集落形成能力、间隙连接细胞间通讯（GJIC）、黏附能力以及细胞表面和内部结构的变化。将细胞接种于裸鼠建立动物肿瘤模型，观察体内肿瘤发生及转移的抑制情况。
BRMS1基因成功转染至A2780细胞系。转染组、阴性对照组和空白对照组细胞生长速率差异无统计学意义（P>0.05）。转染组每孔细胞克隆数为40±4，空白对照组为42±7，阴性对照组为39±4，两两比较差异无统计学意义（P>0.05）。3组细胞S期与G2/M期及G0/G1期比例差异无统计学意义（P>0.05）。电镜检测细胞超微结构显示，转染组GJIC功能高于其他两组。在迁移实验中，转染组、空白对照组和阴性对照组穿膜至下室的细胞数分别为112±23、306±49和322±91，3组间差异有统计学意义（P<0.01）。
BRMS1基因可抑制卵巢癌细胞的体内外转移。
